Recombinant Human DRG1 protein is a Human Full Length protein, in the 1 to 367 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S S T L A K I A E I E A E M A R T Q K N K A T A H H L G L L K A R L A K L R R E L I T P K G G G G G G P G E G F D V A K T G D A R I G F V G F P S V G K S T L L S N L A G V Y S E V A A Y E F T T L T T V P G V I R Y K G A K I Q L L D L P G I I E G A K D G K G R G R Q V I A V A R T C N L I L I V L D V L K P L G H K K I I E N E L E G F G I R L N S K P P N I G F K K K D K G G I N L T A T C P Q S E L D A E T V K S I L A E Y K I H N A D V T L R S D A T A D D L I D V V E G N R V Y I P C I Y V L N K I D Q I S I E E L D I I Y K V P H C V P I S A H H R W N F D D L L E K I W D Y L K L V R I Y T K P K G Q L P D Y T S P V V L P Y S R T T V E D F C M K I H K N L I K E F K Y A L V W G L S V K H N P Q K V G K D H T L E D E D V I Q I V K K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP (PubMed:29915238, PubMed:23711155). Appears to have an intrinsic GTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions (PubMed:23711155). When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation (PubMed:19819225, PubMed:29915238). Binds to microtubules and promotes microtubule polymerization and stability that are required for mitotic spindle assembly during prophase to anaphase transition. GTPase activity is not necessary for these microtubule-related functions (PubMed:28855639).
Alternative names
Developmentally-regulated GTP-binding protein 1, DRG-1, Neural precursor cell expressed developmentally down-regulated protein 3, Translation factor GTPase DRG1, NEDD-3, TRAFAC GTPase DRG1, NEDD3, DRG1
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Recombinant Human DRG1 protein is a Human Full Length protein, in the 1 to 367 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- Q9Y295-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S S T L A K I A E I E A E M A R T Q K N K A T A H H L G L L K A R L A K L R R E L I T P K G G G G G G P G E G F D V A K T G D A R I G F V G F P S V G K S T L L S N L A G V Y S E V A A Y E F T T L T T V P G V I R Y K G A K I Q L L D L P G I I E G A K D G K G R G R Q V I A V A R T C N L I L I V L D V L K P L G H K K I I E N E L E G F G I R L N S K P P N I G F K K K D K G G I N L T A T C P Q S E L D A E T V K S I L A E Y K I H N A D V T L R S D A T A D D L I D V V E G N R V Y I P C I Y V L N K I D Q I S I E E L D I I Y K V P H C V P I S A H H R W N F D D L L E K I W D Y L K L V R I Y T K P K G Q L P D Y T S P V V L P Y S R T T V E D F C M K I H K N L I K E F K Y A L V W G L S V K H N P Q K V G K D H T L E D E D V I Q I V K K
- Accession
- Protein length
Full Length
- Predicted molecular weight
42.7 kDa
- Amino acids
1 to 367
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab109940 was purified using conventional chromatography techniques.
General info
- Function
Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP (PubMed:29915238, PubMed:23711155). Appears to have an intrinsic GTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions (PubMed:23711155). When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation (PubMed:19819225, PubMed:29915238). Binds to microtubules and promotes microtubule polymerization and stability that are required for mitotic spindle assembly during prophase to anaphase transition. GTPase activity is not necessary for these microtubule-related functions (PubMed:28855639).
- Sequence similarities
Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.
- Post-translational modifications
Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The DRG1 protein also known as Developmentally Regulated GTP-binding protein 1 is an important player in cellular functions. This highly conserved protein has a mass of approximately 42 kDa and is expressed in various tissues including the brain and testis indicating its essential role in different biological processes. It contains GTP-binding motifs important for its activity which allows the protein to interact with other molecular partners in the cell facilitating its participation in cellular events.
- Biological function summary
DRG1 engages in several critical cellular processes beyond its mechanical functions. One of its notable roles involves contributions to ribosome biogenesis although it does not form a permanent complex with the ribosome. The protein supports cellular growth and division highlighting its potential in affecting cell cycle regulation. DRG1 associates transiently with other proteins allowing it to influence a range of biological functions including cellular growth signaling pathways.
- Pathways
Research indicates that DRG1 links to important regulatory networks such as the Ras signaling and translational control pathways. Within these pathways DRG1 interacts with proteins like eukaryotic initiation factors which are important for translation regulation. This interaction plays a role in coordinating protein synthesis which supports overall cellular growth and response to environmental cues. Understanding its place in these pathways sheds light on how cells control protein production and growth.
- Associated diseases and disorders
Studies suggest DRG1's involvement in cancer and neurological disorders. In certain cancers altered expression of DRG1 correlates with tumor progression implicating its role in cell proliferation and survival mechanisms. Additionally DRG1 has connections to neurodegenerative disorders potentially through interactions with tau protein which is associated with abnormal protein deposits in the brain. The protein’s link to these diseases highlights the need for further research into its functional mechanisms and potential as a therapeutic target.
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1 product image
SDS-PAGE - Recombinant Human DRG1 protein (ab109940)
15% SDS-PAGE analysis of 3μg ab109940.
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