Recombinant Human DSS1 protein is a Human Full Length protein, in the 1 to 70 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S E K K Q P V D L G L L E E D D E F E E F P A E D W A G L D E D E D A H V W E D N W D D D N V E D D F S N Q L R A E L E K H G Y K M E T S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed:15117943). Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3 (PubMed:22307388). The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells.
Alternative names
C7orf76, DSS1, SHFDG1, SHFM1, SEM1, 26S proteasome complex subunit SEM1, 26S proteasome complex subunit DSS1, Deleted in split hand/split foot protein 1, Split hand/foot deleted protein 1, Split hand/foot malformation type 1 protein
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Recombinant Human DSS1 protein is a Human Full Length protein, in the 1 to 70 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P60896-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S E K K Q P V D L G L L E E D D E F E E F P A E D W A G L D E D E D A H V W E D N W D D D N V E D D F S N Q L R A E L E K H G Y K M E T S
- Accession
- P60896
- Protein length
- Full Length
- Predicted molecular weight
- 10.7 kDa
- Amino acids
- 1 to 70
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab174412 was purified using conventional chromatography.
General info
- Function
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed:15117943). Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3 (PubMed:22307388). The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells.
- Sequence similarities
Belongs to the DSS1/SEM1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
"DSS1" protein also known as Sem1 is a small protein with a molecular mass of approximately 9 kDa. It locates itself in both the nucleus and the cytoplasm of eukaryotic cells. Expressing ubiquitously across various tissues DSS1 plays an essential role in different cellular processes. Its compact size allows flexibility enabling interactions with numerous cellular machinery components.
- Biological function summary
DSS1 interacts with several proteins within the cell. It is an integral part of the 26S proteasome and the BRCA2 complex both critical for DNA repair and protein degradation pathways. Through these associations DSS1 contributes to maintaining genomic stability by assisting in the DNA repair processes. The presence in such complexes indicates its multifaceted role in cellular regulation and continuity.
- Pathways
DSS1 plays a role in the homologous recombination repair and ubiquitin-proteasome pathways. Its interaction with BRCA2 highlights its importance in the repair of DNA double-strand breaks. In the ubiquitin-proteasome system DSS1 partners with proteins like Rpn3 and Rpn7 influencing degradation of misfolded or damaged proteins ensuring cellular proteostasis.
- Associated diseases and disorders
DSS1 shows a significant connection to cancer and Fanconi anemia. Its association with BRCA2 links it to breast and ovarian cancer susceptibility as defects in this complex impair DNA repair. Additionally abnormalities in DSS1 or its related pathways can contribute to Fanconi anemia where the DNA repair machinery is compromised. Understanding these roles further emphasizes the importance of DSS1 in cellular health and pathology.
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SDS-PAGE - Recombinant Human DSS1 protein (ab174412)
15% SDS-PAGE analysis of ab174412 (3 μg).
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