Recombinant Human DSTN protein is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M A S G V Q V A D E V C R I F Y D M K V R K C S T P E E I K K R K K A V I F C L S A D K K C I I V E E G K E I L V G D V G V T I T D P F K H F V G M L P E K D C R Y A L Y D A S F E T K E S R K E E L M F F L W A P E L A P L K S K M I Y A S S K D A I K K K F Q G I K H E C Q A N G P E D L N R A C I A E K L G G S L I V A F E G C P V L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
Alternative names
ACTDP, DSN, DSTN, Destrin, Actin-depolymerizing factor, ADF
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Recombinant Human DSTN protein is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P60981-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A S G V Q V A D E V C R I F Y D M K V R K C S T P E E I K K R K K A V I F C L S A D K K C I I V E E G K E I L V G D V G V T I T D P F K H F V G M L P E K D C R Y A L Y D A S F E T K E S R K E E L M F F L W A P E L A P L K S K M I Y A S S K D A I K K K F Q G I K H E C Q A N G P E D L N R A C I A E K L G G S L I V A F E G C P V L E H H H H H H
- Accession
- P60981
- Protein length
- Full Length
- Predicted molecular weight
- 19.5 kDa
- Amino acids
- 1 to 165
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab128429 was purified using conventional chromatography techniques.
General info
- Function
Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
- Sequence similarities
Belongs to the actin-binding proteins ADF family.
- Post-translational modifications
ISGylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Destrin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DSTN also known as destrin or actin depolymerizing factor is an actin-binding protein with a relatively low molecular mass of approximately 19 kDa. DSTN plays a significant role in actin filament dynamics by enhancing the disassembly of actin filaments into monomeric actin components thereby regulating cytoskeleton organization. The protein is present in a wide range of tissues including the brain and skeletal muscles indicating its importance in various physiological processes.
- Biological function summary
DSTN regulates actin cytoskeleton remodeling impacting cell shape and motility. It acts by binding to actin enhancing the turnover and dismantling of actin filaments which is critical for maintaining cellular architecture. DSTN is not known to be part of a larger protein complex but it interacts with other actin-related proteins to exert its function efficiently. Its role in cell movement and structure integrates it into larger cellular processes such as cell division migration and potentially signal transduction.
- Pathways
Cytoskeletal rearrangement and cell motility highlight the involvement of DSTN. DSTN associates closely with the Rho and Rac signaling pathways pivotal for cytoskeletal changes and cell division processes. Through these pathways DSTN contributes to modulating cellular responses by interacting with proteins like cofilin another actin-binding protein. This interaction with signaling pathways highlights DSTN's broader influence on cellular functions.
- Associated diseases and disorders
DSTN has an association with neurodegenerative diseases and cancer progression. Aberrations in its activity can result in cytoskeletal dysfunctions to which disorders such as Alzheimer’s disease relate due to the importance of cytoskeletal integrity in neuron function. Additionally DSTN's involvement in cell motility links it to cancer metastasis where unchecked cell movement promotes tumor spread. In these diseases DSTN collaborates mutably with the tau protein and other cytoskeletal regulators highlighting DSTN's relevance in pathological conditions.
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SDS-PAGE - Recombinant Human DSTN protein (ab128429)
15% SDS-PAGE analysis of 3µg ab128429.
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