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AB137172

Recombinant Human DTD1 protein

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Recombinant Human DTD1 protein is a Human Full Length protein, in the 1 to 209 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C20orf88, DUEB, HARS2, DTD1, D-aminoacyl-tRNA deacylase 1, DTD, DNA-unwinding element-binding protein B, Gly-tRNA(Ala) deacylase, Histidyl-tRNA synthase-related, DUE-B

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SDS-PAGE - Recombinant Human DTD1 protein (AB137172)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DTD1 protein (AB137172)

15% SDS-PAGE analysis of ab137172 (3μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q8TEA8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFHLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP","proteinLength":"Full Length","predictedMolecularWeight":"25.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":209,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TEA8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DTD1 also known as D-Tyr-tRNA deacylase is a protein involved mechanically in detoxifying D-amino acids that mistakenly attach to tRNA molecules. It functions by hydrolyzing the ester bond between the D-amino acid and tRNA preventing incorporation during protein synthesis. The protein has a relatively small mass of approximately 20 kilodaltons. DTD1 is expressed in various tissues with notable expression in the liver and kidney where clearing abnormal peptides is critical.
Biological function summary

The removal of incorrect D-amino acids ensures fidelity in protein translation. DTD1 functions not only as a single enzyme but associates with other molecules in multi-enzyme complexes. These complexes help maintain the accuracy of protein biosynthesis across different cellular environments. By doing so DTD1 contributes to cellular homeostasis and conserves the correct folding and function of proteins necessary for normal cellular activity.

Pathways

DTD1 plays a significant role in aminoacyl-tRNA biosynthesis and interaction pathways. It collaborates with proteins like tRNA synthetases in the aminoacyl-tRNA synthesis pathway ensuring only L-amino acids are incorporated into growing polypeptide chains. This pathway is essential for high-fidelity protein translation which allows cells to synthesize functional proteins efficiently affecting overall protein homeostasis.

Mismanagement of D-amino acids can lead to neurodegenerative diseases and metabolic disorders. For example abnormal D-amino acid levels have been linked to disorders like Alzheimer's disease. In such conditions DTD1's activity or lack thereof can influence the progression of the disease. Additionally interactions with other related proteins like L-amino acid oxidase can impact disease pathways as imbalances in these systems contribute to disease states.
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Specifications

Form

Liquid

Additional notes

ab137172 was purified using conventional chromatography.

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General info

Function

Possible ATPase (PubMed : 15653697) involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes (PubMed : 20065034).. An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.

Sequence similarities

Belongs to the DTD family.

Post-translational modifications

Preferentially phosphorylated in cells arrested early in S phase (PubMed:15653697). Phosphorylation in the C-terminus weakens the interaction with CDC45 (PubMed:20065034).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Possible ATPase (PubMed : 15653697) involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes (PubMed : 20065034).. An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
See full target information DTD1
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