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AB165631

Recombinant Human DTX3L protein (GST tag N-Terminus)

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Recombinant Human DTX3L protein (GST tag N-Terminus) is a Human Fragment protein, in the 3 to 110 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

BBAP, DTX3L, E3 ubiquitin-protein ligase DTX3L, B-lymphoma- and BAL-associated protein, Protein deltex-3-like, RING-type E3 ubiquitin transferase DTX3L, Rhysin-2, Rhysin2

1 Images
SDS-PAGE - Recombinant Human DTX3L protein (GST tag N-Terminus) (AB165631)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DTX3L protein (GST tag N-Terminus) (AB165631)

ab165631 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8TDB6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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SDS-PAGE - Recombinant Human ZNF358 protein (GST tag N-Terminus) (AB165455)

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AB165467

Recombinant Human ZBTB46 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZBTB46 protein (GST tag N-Terminus) (AB165467)

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AB177595

Recombinant Human TCF-4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TCF-4 protein (His tag N-Terminus) (AB177595)

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Primary Antibodies

AB70621

Anti-DTX3L antibody

Immunoprecipitation - Anti-DTX3L antibody (AB70621)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKKGEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAE","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":110,"aminoAcidStart":3,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8TDB6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed : 12670957, PubMed : 19818714, PubMed : 23230272, PubMed : 26479788). Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed : 28525742). In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed : 19818714). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed : 19818714). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed : 23230272). By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed : 26479788). Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed : 24790097). In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed : 24790097). In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteasomal-mediated degradation (PubMed : 26479788).

Sequence similarities

Belongs to the Deltex family.

Post-translational modifications

Autoubiquitinated.

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Product protocols

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Target data

E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed : 12670957, PubMed : 19818714, PubMed : 23230272, PubMed : 26479788). Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed : 28525742). In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed : 19818714). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed : 19818714). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed : 23230272). By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed : 26479788). Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed : 24790097). In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed : 24790097). In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteasomal-mediated degradation (PubMed : 26479788).
See full target information DTX3L
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