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AB109838

Recombinant Human DTYMK protein

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(2 Publications)

Recombinant Human DTYMK protein is a Human Full Length protein, in the 1 to 212 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CDC8, TMPK, TYMK, DTYMK, Thymidylate kinase, dTMP kinase

1 Images
SDS-PAGE - Recombinant Human DTYMK protein (AB109838)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DTYMK protein (AB109838)

SDS-PAGE analysis of Recombinant Human DTYMK protein (ab109838), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human DTYMK protein

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P23919

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.04% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK","proteinLength":"Full Length","predictedMolecularWeight":"24 kDa","actualMolecularWeight":null,"aminoAcidEnd":212,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23919","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

No detergents such as urea, Triton or Tween have been used to purify the protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).

Sequence similarities

Belongs to the thymidylate kinase family.

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Product protocols

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Target data

Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).
See full target information DTYMK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 19:823-7 PubMed2017365

1991

Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

J Y Su,R A Sclafani

Cancer research 40:1235-9 PubMed6244089

1980

Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver.

Applications

Unspecified application

Species

Unspecified reactive species

R R Cummins,D Balinsky
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