Recombinant Human Dynein light chain protein is a Human Full Length protein, in the 1 to 105 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G E T E G K K D E A D Y K R L Q T F P L V R H S D M P E E M R V E T M E L C V T A C E K F S N N N E S A A K M I K E T M D K K F G S S W H V V I G E G F G F E I T H E V K N L L Y L Y F G G T L A V C V W K C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity (By similarity).
Alternative names
Dynein axonemal light chain 4, DNAL4
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Recombinant Human Dynein light chain protein is a Human Full Length protein, in the 1 to 105 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O96015-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G E T E G K K D E A D Y K R L Q T F P L V R H S D M P E E M R V E T M E L C V T A C E K F S N N N E S A A K M I K E T M D K K F G S S W H V V I G E G F G F E I T H E V K N L L Y L Y F G G T L A V C V W K C S
- Accession
- O96015
- Protein length
- Full Length
- Predicted molecular weight
- 14.1 kDa
- Amino acids
- 1 to 105
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab107140 is purified using conventional chromatography techniques.
General info
- Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity (By similarity).
- Sequence similarities
Belongs to the dynein light chain family.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dynein light chain also known as DLC is a component of the dynein protein complex. This complex functions as a motor protein driving cellular transport by walking along microtubules using ATP hydrolysis. Dynein light chain is small approximately 8 kDa in mass yet it plays an integral role in dynein’s mechanical operation. These proteins are ubiquitously expressed throughout various tissues in eukaryotic organisms including humans where they assist in the intracellular transport of organelles proteins and RNA.
- Biological function summary
Dynein light chain is essential for the function and stability of the dynein motor complex which also includes heavy chains intermediate chains and other light chains. The dynein complex mediates the retrograde transport along axons and is involved in cell division by contributing to spindle positioning. Dynein light chain binds to cargo adaptor proteins and helps the dynein complex anchor to various cellular structures ensuring targeted transport within the cell.
- Pathways
The dynein light chain plays an important role in the intracellular transport pathway particularly in vesicle trafficking and organelle positioning. It interacts with key proteins such as dynactin an important cofactor that enhances dynein's motor function and cargo binding. Additionally dynein light chain participates in mitotic spindle orientation collaborating with proteins like LIS1 to ensure proper chromosome segregation during mitosis.
- Associated diseases and disorders
Dysregulation of dynein light chain function is linked to neurodegenerative conditions such as Huntington's disease and motor neuron disorders. Alterations in dynein-mediated transport can lead to the accumulation of protein aggregates and impaired neural function. In Huntington’s disease this disruption affects the dynein and dynactin interaction contributing to neuronal dysfunction and cell death. Dynein light chain’s efficiency and integrity also intersect with LIS1 in lissencephaly where reduced dynein activity can result in abnormal neuronal migration and brain structure abnormalities.
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1 product image
SDS-PAGE - Recombinant Human Dynein light chain protein (ab107140)
15% SDS-PAGE showing ab107140 (3 μg) at approximately 14.1 kDa.
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