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AB114522

Recombinant Human DYRK1A protein (GST tag N-Terminus)

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Recombinant Human DYRK1A protein (GST tag N-Terminus) is a Human Fragment protein, in the 674 to 763 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

DYRK, MNB, MNBH, DYRK1A, Dual specificity tyrosine-phosphorylation-regulated kinase 1A, Dual specificity YAK1-related kinase, HP86, Protein kinase minibrain homolog, hMNB

1 Images
SDS-PAGE - Recombinant Human DYRK1A protein (GST tag N-Terminus) (AB114522)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DYRK1A protein (GST tag N-Terminus) (AB114522)

12.5% SDS-PAGE analysis of Human DYRK1A protein (ab114522). Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q13627

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB259869

Anti-DYRK1A antibody [EPR24132-61]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-DYRK1A antibody [EPR24132-61] (AB259869)

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Proteins & Peptides

AB316501

Recombinant Human CD3 Epsilon Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD3 Epsilon Protein Standard (His tag) (AB316501)

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Proteins & Peptides

AB276616

Recombinant Human CD81 protein (His tag)

SDS-PAGE - Recombinant Human CD81 protein (His tag) (AB276616)

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Proteins & Peptides

AB316459

Recombinant Human FGL1 Protein Standard

Sandwich ELISA - Recombinant Human FGL1 Protein Standard (AB316459)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"NQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVAS","proteinLength":"Fragment","predictedMolecularWeight":"35.53 kDa","actualMolecularWeight":null,"aminoAcidEnd":763,"aminoAcidStart":674,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q13627","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities (PubMed : 20981014, PubMed : 21127067, PubMed : 23665168, PubMed : 30773093, PubMed : 8769099). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3 (PubMed : 23665168). Plays an important role in double-strand breaks (DSBs) repair following DNA damage (PubMed : 31024071). Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR) (PubMed : 30773093). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 25620562, PubMed : 29849146). May play a role in a signaling pathway regulating nuclear functions of cell proliferation (PubMed : 14500717). Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Has pro-survival function and negatively regulates the apoptotic process (By similarity). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1 (By similarity). This in turn inhibits p53/TP53 activity and apoptosis (By similarity). Phosphorylates SEPTIN4, SEPTIN5 and SF3B1 at 'Thr-434' (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

Post-translational modifications

Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities (PubMed : 20981014, PubMed : 21127067, PubMed : 23665168, PubMed : 30773093, PubMed : 8769099). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3 (PubMed : 23665168). Plays an important role in double-strand breaks (DSBs) repair following DNA damage (PubMed : 31024071). Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR) (PubMed : 30773093). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 25620562, PubMed : 29849146). May play a role in a signaling pathway regulating nuclear functions of cell proliferation (PubMed : 14500717). Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Has pro-survival function and negatively regulates the apoptotic process (By similarity). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1 (By similarity). This in turn inhibits p53/TP53 activity and apoptosis (By similarity). Phosphorylates SEPTIN4, SEPTIN5 and SF3B1 at 'Thr-434' (By similarity).
See full target information DYRK1A
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