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AB87462

Recombinant Human Dysbindin protein (His tag N-Terminus)

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Recombinant Human Dysbindin protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 270 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

BLOC1S8, My031, DTNBP1, Dysbindin, Biogenesis of lysosome-related organelles complex 1 subunit 8, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein, BLOC-1 subunit 8, HPS7 protein

2 Images
SDS-PAGE - Recombinant Human Dysbindin protein (AB87462)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Dysbindin protein (AB87462)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

SDS-PAGE - Recombinant Human Dysbindin protein (AB87462)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Dysbindin protein (AB87462)

ab87462 on 15% SDS-PAGE (3μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96EV8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTANLTHLEASFEEVENNLLHLEDLCGQCELERCKHMQSQQLENYKKNKRKELETFKAELDAEHAQKVLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAERREPIGSMSSMEVNVDMLEQMDLMDISDQEALDVFLNSGGEENTVLSPALGPESSTCQNEITLQVPNPSELRAKPPSSSSTCTDSATRDISEGGESPVVQSDEEEVQVDTALATSHTDREATPDGGEDSDS","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":270,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96EV8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dysbindin also known as Dystrobrevin Binding Protein 1 (DTNBP1) is an important protein that plays an important role in cellular processes. Its molecular weight is approximately 40 kDa. Dysbindin is expressed in a variety of tissues but it shows higher expression in the brain and testis. Within cells dysbindin localizes to the cytoplasm and is notably present at synaptic sites where it assumes an important function in neuronal communication.
Biological function summary

Multiple cellular processes involve dysbindin including synaptic transmission and intracellular signaling. Dysbindin is a component of the Biogenesis of Lysosome-related Organelles Complex 1 (BLOC-1) which is important for the biogenesis and trafficking of lysosome-related organelles. Its role in ensuring proper lysosomal function is linked to the regulation of synaptic vesicle release influencing neurotransmitter availability in synaptic clefts.

Pathways

Several important biological networks engage dysbindin particularly affecting neural and cellular functions. It participates in the synaptic vesicle cycle and pigment cell pathways. Dysbindin’s functional relationship with proteins such as Snapin and Pallidin illustrates its connection to BLOC-1-related pathways indicating its integrative role in synaptic endosome trafficking and pigment gene expression processes.

Dysbindin has a significant association with schizophrenia and Hermansky-Pudlak syndrome type 7. Genetic studies have linked the dysbindin gene to altered risk for schizophrenia through its impact on synaptic function and plasticity. Additionally its interaction with other BLOC-1 components like Pallidin ties it to Hermansky-Pudlak syndrome where disrupted organelle trafficking affects pigmentation and leads to systemic symptoms.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography.

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General info

Function

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway.

Sequence similarities

Belongs to the dysbindin family.

Post-translational modifications

Ubiquitinated by TRIM32. Ubiquitination leads to DTNBP1 degradation.. Isoforms 1 and 2 highly phosphorylated by PRKDC in vitro. Isoform 3 only weakly phosphorylated by PRKDC in vitro.

Subcellular localisation

Endosome membrane

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Product protocols

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Target data

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway.
See full target information DTNBP1
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Complementary Products

Primary Antibodies

AB124967

Anti-Dysbindin antibody [EPR7042(B)]

RabMAb|Recombinant

Western blot - Anti-Dysbindin antibody [EPR7042(B)] (AB124967)

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