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AB82207

Recombinant Human E2F1 protein

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(4 Publications)

Recombinant Human E2F1 protein is a Human Full Length protein, expressed in Baculovirus infected insect cells, with >95%, suitable for SDS-PAGE, GSA, EMSA, FuncS.

View Alternative Names

RBBP3, E2F1, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1, RBAP-1, RBBP-3

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected insect cells

Tags

His tag

Applications

SDS-PAGE, FuncS, GSA, EMSA

applications

Biologically active

No

Accession

Q01094

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "GSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EMSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>1 unit is sufficient in a 20 µl reaction.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(1 unit equals 1 ng of purified protein)100 units in protein-protein interaction assays;50 untis in <i>in vitro</i> transcription, DNA binding and DNA bending assays;At an assay dependent dilution in cell growth and proliferation assays .</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q01094","tags":[{"tag":"His","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E2F1 protein also known as E2F transcription factor 1 is an important regulator of the cell cycle. It has a molecular weight of approximately 47 kDa. E2F1 protein is expressed in many tissues with higher expression levels in proliferating cells. It often acts as a transcription factor by binding to specific DNA sequences controlling the transition of cells from G1 to S phase of the cell cycle. This role is vital for the proper progression of the cycle and cellular proliferation.
Biological function summary

E2F1 plays a significant role in cell growth regulation and apoptosis. E2F1 functions within a complex often forming heterodimers with DP proteins. This combination enhances its ability to bind DNA and activate transcription of genes necessary for DNA replication and cell cycle progression. E2F1 also participates in the control of apoptosis by regulating the expression of pro-apoptotic genes balancing cell proliferation and death.

Pathways

E2F1 is deeply embedded in cell cycle regulatory pathways and the p53 signaling pathway. It closely interacts with proteins like RB (Retinoblastoma protein) and p53. RB protein regulates E2F1 activity by controlling its release while p53 helps in mediating the cell's response to DNA damage potentially leading to either cell cycle arrest or apoptosis. These interactions highlight E2F1's role in maintaining cellular homeostasis.

E2F1 is often linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of E2F1 can lead to uncontrolled cell proliferation contributing to oncogenesis. In the context of neurodegenerative diseases E2F1 influences neuronal apoptosis and may be associated with disorders such as Alzheimer's. E2F1 interacts with other proteins such as p53 in cancer where it affects both cell cycle dynamics and apoptosis illustrating its complex involvement in disease progression.
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Specifications

Form

Liquid

Additional notes

Purified by a combination of affinity chromatography and FPLC chromatography.

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General info

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed : 10675335, PubMed : 12717439, PubMed : 17050006, PubMed : 17704056, PubMed : 18625225, PubMed : 28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed : 8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed : 20176812). Directly activates transcription of PEG10 (PubMed : 17050006, PubMed : 18625225, PubMed : 28992046). Positively regulates transcription of RRP1B (PubMed : 20040599).

Sequence similarities

Belongs to the E2F/DP family.

Post-translational modifications

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase (PubMed:12717439, PubMed:7838523). Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis (PubMed:12717439). Phosphorylation at Ser-403 by GSK3B promotes interaction with USP11, leading to its deubiquitination and stabilization (PubMed:28992046).. Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation (PubMed:28992046). Deubiquitinated by USP11 following phosphorylation by GSK3B, promoting its stability (PubMed:28992046).. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.. Methylation at Lys-185 by SETD7 promotes E2F1 ubiquitin-dependent proteasomal degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed : 10675335, PubMed : 12717439, PubMed : 17050006, PubMed : 17704056, PubMed : 18625225, PubMed : 28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed : 8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed : 20176812). Directly activates transcription of PEG10 (PubMed : 17050006, PubMed : 18625225, PubMed : 28992046). Positively regulates transcription of RRP1B (PubMed : 20040599).
See full target information E2F1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115608 PubMed40279246

2025

LncRNA SLNCR phenocopies the E2F1 DNA binding site to promote melanoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kushani Shah,Eleni Anastasakou,Leinal Sejour,Yufei Wang,Leon Wert-Lamas,Christopher Rauchet,Sabine Studer,Simon Goller,Robert J Distel,Wayne Marasco,Lalith Perera,Ioannis S Vlachos,Carl D Novina

Cell reports. Medicine 6:101966 PubMed39970873

2025

KRAS-driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyan Jiang,Tao Wang,Bin Zhao,Haonan Sun,Yuman Dong,Yong Ma,Zhigang Li,Yuxia Wu,Keshen Wang,Xiaoying Guan,Bo Long,Long Qin,Wengui Shi,Lei Shi,Qichen He,Wenbo Liu,Mingdou Li,Lixia Xiao,Chengliang Zhou,Hui Sun,Jing Yang,Junhong Guan,Huinian Zhou,Zeyuan Yu,Zuoyi Jiao

The Journal of international medical research 51:3000605231163780 PubMed36994850

2023

hMSH2 coordinated with the expression of E2F1 promotes platinum response in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qian Hu,Bao-Ying Zhang,Tian Hua

Nature communications 12:3337 PubMed34099689

2021

High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ludovica Vanzan,Hadrien Soldati,Victor Ythier,Santosh Anand,Simon M G Braun,Nicole Francis,Rabih Murr
View all publications
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