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AB152352

Recombinant Human E2F4 protein (GST tag N-Terminus)

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Recombinant Human E2F4 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 413 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Transcription factor E2F4, E2F-4, E2F4

1 Images
SDS-PAGE - Recombinant Human E2F4 protein (GST tag N-Terminus) (AB152352)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human E2F4 protein (GST tag N-Terminus) (AB152352)

12.5% SDS-PAGE analysis of ab152352 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q16254

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB150360

Anti-E2F4 antibody [EPR8259]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (AB150360)

  • 2
  • 3
Primary Antibodies

AB174847

Anti-Caspase-14 antibody [EPR12927]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

  • 4
  • 1
Cell Lines & Lysates

AB257932

Human E2F4 knockout HEK-293T cell lysate

Western blot - Human E2F4 knockout HEK-293T cell lysate (AB257932)

  • 0
  • 0
Cell Lines & Lysates

AB266119

Human E2F4 knockout HEK-293T cell line

Advanced Validation

Western blot - Human E2F4 knockout HEK-293T cell line (AB266119)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL","proteinLength":"Full Length","predictedMolecularWeight":"70.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":413,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q16254","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F4 sometimes known as E2F-4 is part of the E2F family of transcription factors. It plays a critical role in controlling the cell cycle by regulating the expression of genes necessary for DNA synthesis. E2F4 has a molecular weight of around 45 kDa. These transcription factors are widely expressed in a variety of tissues reflecting their importance in cell biology. E2F4 typically functions by forming complexes with dimerization partner (DP) proteins mainly in the nucleus where it binds to DNA and influences transcription.
Biological function summary

E2F4 functions as a regulator of cell cycle progression. It forms complexes localizing mostly in the nucleus controlling the transition from G1 to S phase by regulating genes associated with cell cycle arrest and DNA replication. Partnering with members of the DP family enhances its DNA binding capacity influencing genes involved in cell cycle arrest and DNA repair processes. E2F4 predominantly acts as a transcriptional repressor in quiescent and differentiating cells maintaining the cell in a non-proliferative state.

Pathways

E2F4 is significant in the regulation of the cell cycle and DNA damage response pathways. It engages with the retinoblastoma protein family (pRB p107 p130) to exert repressive effects on E2F-target genes ensuring proper cell cycle exit and maintaining the G0 phase. The interaction with p130 is instrumental during the G0 state preventing inappropriate cell proliferation and contributing to the maintenance of cellular quiescence.

E2F4's role is noteworthy in cancer and retinoblastoma. Alterations in its regulation or activity can lead to unchecked cell proliferation a hallmark of cancerous growths. The malfunction or suppression of its activity can disrupt cell cycle control contributing to oncogenesis. In instances of retinoblastoma improper interaction between E2F4 and the retinoblastoma protein family can result in disrupted cell cycle checkpoints highlighting its importance in maintaining cellular homeostasis and preventing tumorigenesis.
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Specifications

Form

Liquid

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General info

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation : together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Sequence similarities

Belongs to the E2F/DP family.

Post-translational modifications

Differentially phosphorylated in vivo.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation : together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
See full target information E2F4
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Product promise

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