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AB114573

Recombinant Human E2F6 protein

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Recombinant Human E2F6 protein is a Human Full Length protein, in the 1 to 281 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Transcription factor E2F6, E2F-6, E2F6

1 Images
SDS-PAGE - Recombinant Human E2F6 protein (AB114573)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human E2F6 protein (AB114573)

12.5% SDS-PAGE Stained with Coomassie Blue

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

O75461

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein</p>" } } }
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Sequence info

[{"sequence":"MSQQRPARKLPSLLLDPTEETVRRRCRDPINVEGLLPSKIRINLEDNVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYDITNVLDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN","proteinLength":"Full Length","predictedMolecularWeight":"56.65 kDa","actualMolecularWeight":null,"aminoAcidEnd":281,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O75461","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E2F6 a member of the E2F family of transcription factors acts as a transcriptional repressor. It regulates genes essential for cell cycle progression particularly those involved in DNA replication. E2F6 lacks the transactivation domain present in other E2F family members allowing it uniquely to repress transcription without activating it. E2F6 has a molecular mass of approximately 30 kDa. The protein is mostly expressed in the nucleus of proliferating cells but it is also found in certain other tissues including the brain and testes.
Biological function summary

E2F6 plays an important role in regulating the cell cycle. It belongs to a multiprotein complex called the repressive E2F6 complex or PRC1 (Polycomb Repressive Complex 1). This complex is responsible for chromatin remodeling and transcriptional repression of target genes important in S phase entry and mitosis. By repressing specific E2F-responsive genes E2F6 inhibits excessive cell proliferation therefore maintaining proper cell cycle control.

Pathways

E2F6 contributes to important pathways like the cell cycle and DNA damage response pathways. In these pathways it is involved in controlling the transition phases of the cell cycle particularly regulating entry into the S phase. E2F6 interacts with proteins such as Rb (retinoblastoma protein) and other E2F members like E2F1 and E2F4 to control cell growth and ensure genome integrity.

Dysregulation of E2F6 has links to cancer and developmental disorders. In cancer overexpression or mutations affecting E2F6 can lead to uncontrolled cell proliferation due to improper repression of cell cycle genes. Additionally defects in the E2F6 gene have associations with developmental syndromes through interactions with other proteins like EZH2 a component of the Polycomb group proteins. These associations highlight the importance of E2F6 in both normal cell function and disease states.
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Specifications

Form

Liquid

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General info

Function

Inhibitor of E2F-dependent transcription (PubMed : 9501179, PubMed : 9689056, PubMed : 9704927). Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed : 9501179). Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed : 9501179). E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed : 9501179, PubMed : 9704927). Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed : 9501179, PubMed : 9689056). Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity). May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed : 9501179).

Sequence similarities

Belongs to the E2F/DP family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Inhibitor of E2F-dependent transcription (PubMed : 9501179, PubMed : 9689056, PubMed : 9704927). Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed : 9501179). Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed : 9501179). E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed : 9501179, PubMed : 9704927). Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed : 9501179, PubMed : 9689056). Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity). May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed : 9501179).
See full target information E2F6
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