Recombinant Human E2F8 protein is a Human Full Length protein, in the 1 to 431 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
Amino acid sequence
M A Q L A A I C K M Q L E E Q S S E S R Q K V K V Q L A R S G P C K P V A P L D P P V N A E M E L T A P S L I Q P L G M V P L I P S P L S S A V P L I L P Q A P S G P S Y A I Y L Q P T Q A H Q S V T P P Q G L S P T V C T T H S S K A T G S K D S T D A T T E K A A N D T S K A S A S T R P G S L L P A P E R Q G A K S R T R E P A G E R G S K R A S M L E D S G S K K K F K E D L K G L E N V S A T L F P S G Y L I P L T Q C S S L G A E S I L S G K E N S S A L S P N H R I Y S S P I A G V I P V T S S E L T A V N F P S F H V T P L K L M V S P T S V A A V P V G N S P A L A S S H P V P I Q N P S S A I V N F T L Q H L G L I S P N V Q L S A S P G S G I V P V S P R I E S V N V A P E N A G T Q Q G R A T N Y D S P V P G Q S Q P N G Q S V A V T G A Q Q P V P V T P K G S Q L V A E S F F R T P G G P T K P T S S S C M D F E G A N K T S L G T L F V P Q R K L E V S T E D V H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.
Alternative names
Transcription factor E2F8, E2F-8, E2F8
Recommended products
Recombinant Human E2F8 protein is a Human Full Length protein, in the 1 to 431 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Alternative names
Transcription factor E2F8, E2F-8, E2F8
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
- Accession
- A0AVK6-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M A Q L A A I C K M Q L E E Q S S E S R Q K V K V Q L A R S G P C K P V A P L D P P V N A E M E L T A P S L I Q P L G M V P L I P S P L S S A V P L I L P Q A P S G P S Y A I Y L Q P T Q A H Q S V T P P Q G L S P T V C T T H S S K A T G S K D S T D A T T E K A A N D T S K A S A S T R P G S L L P A P E R Q G A K S R T R E P A G E R G S K R A S M L E D S G S K K K F K E D L K G L E N V S A T L F P S G Y L I P L T Q C S S L G A E S I L S G K E N S S A L S P N H R I Y S S P I A G V I P V T S S E L T A V N F P S F H V T P L K L M V S P T S V A A V P V G N S P A L A S S H P V P I Q N P S S A I V N F T L Q H L G L I S P N V Q L S A S P G S G I V P V S P R I E S V N V A P E N A G T Q Q G R A T N Y D S P V P G Q S Q P N G Q S V A V T G A Q Q P V P V T P K G S Q L V A E S F F R T P G G P T K P T S S S C M D F E G A N K T S L G T L F V P Q R K L E V S T E D V H
- Accession
- Protein length
Full Length
- Amino acids
1 to 431
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.
- Sequence similarities
Belongs to the E2F/DP family.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
- Activity summary
E2F8 also known as transcription factor E2F8 plays a vital role in cell cycle regulation. This protein has a molecular weight of approximately 97 kDa. E2F8 is widely expressed in various tissues with significant levels detected in the liver and placenta. As part of the E2F family it functions as a transcription factor repressing or promoting the transcription of target genes involved in cell proliferation.
- Biological function summary
E2F8 acts as a regulator within the context of the cell cycle. It forms a complex with other E2F family members such as E2F7 which can bind to specific DNA sequences and regulate the transcription of genes necessary for the G1/S transition. This activity makes E2F8 essential for controlling cell growth and maintaining cellular homeostasis. E2F8's ability to act as a repressor distinguishes it from other E2Fs which typically activate transcription.
- Pathways
E2F8 integrates into the mechanistic framework of the cell cycle and DNA repair pathways. Its activity is closely tied to the p53 pathway where it can influence p53-mediated transcriptional repression. E2F8 influences other family proteins like E2F1 which are important in driving cell cycle progression and apoptosis. By modulating these pathways E2F8 fine-tunes the balance between cell division and cell death—an important aspect of normal development and tumor suppression.
- Associated diseases and disorders
E2F8 has a notable connection with cancer and developmental disorders. Its dysregulation has been observed in various cancers such as hepatocellular carcinoma where it can promote oncogenic transformation by altering cell cycle dynamics. E2F8 also interacts with proteins like CCNE1 which is often overexpressed in tumors. Furthermore abnormalities in E2F8 function can contribute to birth defects by disrupting normal cell cycle control during embryonic development.
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1 product image
SDS-PAGE - Recombinant Human E2F8 protein (ab153513)
ab153513 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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