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AB128434

Recombinant Human EAP30 protein

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Recombinant Human EAP30 protein is a Human Full Length protein, in the 1 to 258 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

EAP30, SNF8, Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22

1 Images
SDS-PAGE - Recombinant Human EAP30 protein (AB128434)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EAP30 protein (AB128434)

Analysis of ab128434 (Human EAP30 protein) on a 15% SDS-PAGE gel : Lane 1 : MW marker
Lane 2 ab128434 at 3 μg

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96H20

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP","proteinLength":"Full Length","predictedMolecularWeight":"31.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96H20","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EAP30 also known as VPS22 or SNF8 is a component of the ESCRT-II complex with a mass of approximately 30 kDa. It plays an important role in the endosomal sorting process a mechanism that directs the transport of ubiquitinated transmembrane proteins for lysosomal degradation. EAP30 is expressed in various tissues but shows high expression levels in cells involved in membrane trafficking and processes requiring active protein sorting.
Biological function summary

The EAP30 protein functions as part of the ESCRT-II complex which is essential for the formation of multivesicular bodies (MVBs) and their sorting machinery. EAP30 interacts with other components of the ESCRT machinery such as EAP20 and VPS36 ensuring effective processing of cellular signaling receptors and achieving membrane dynamics. This interaction facilitates the correct trafficking of membrane proteins which is necessary for maintaining cellular homeostasis.

Pathways

EAP30 is deeply integrated into the endosomal-lysosomal pathway which is essential for directing proteins toward lysosomal degradation. The protein operates closely with the ESCRT complex including ESCRT-I and ESCRT-III and is involved in endocytosis and receptor downregulation. It coordinates with proteins like VPS4 during late stages of vesicle budding and abscission essential for accurate vesicle scission events.

Malfunctions in EAP30 activities can relate to neurodegenerative conditions such as Alzheimer's disease and frontotemporal dementia. EAP30's incorrect function could impair protein degradation processes leading to the accumulation of ubiquitinated proteins. The protein also shows connections with components like TSG101 which further roles in vesicular transport perturbations seen in these diseases indicating possible therapeutic targets for intervention strategies.
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Specifications

Form

Liquid

Additional notes

Purified by conventional chromatography techniques.

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General info

Function

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs, and plays a role in autophagy (PubMed : 38423010). The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413).

Sequence similarities

Belongs to the SNF8 family.

Subcellular localisation

Endosome membrane

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Product protocols

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Target data

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs, and plays a role in autophagy (PubMed : 38423010). The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413).
See full target information SNF8
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