Recombinant Human EB2 protein is a Human protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P G P T Q T L S P N G E N N N D I I Q D N N G T I I P F R K H T V R G E R S Y S W G M A V N V Y S T S I T Q E T M S R H D I I A W V N D I V S L N Y T K V E Q L C S G A A Y C Q F M D M L F P G C I S L K K V K F Q A K L E H E Y I H N F K L L Q A S F K R M N V D K V I P V E K L V K G R F Q D N L D F I Q W F K K F Y D A N Y D G K E Y D P V E A R Q G Q D A I P P P D P G E Q I F N L P K K S H H A N S P T A G A A K S S P A A K P G S T P S R P S S A K R A S S S G S A S K S D K D L E T Q V I Q L N E Q V H S L K L A L E G V E K E R D F Y F G K L R E I E L L C Q E H G Q E N D D L V Q R L M D I L Y A S E E H E G H T E E P E A E E Q A H E Q Q P P Q Q E E Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).
Alternative names
RP1, MAPRE2, Microtubule-associated protein RP/EB family member 2, APC-binding protein EB2, End-binding protein 2, EB2
Recommended products
Recombinant Human EB2 protein is a Human protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q15555-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P G P T Q T L S P N G E N N N D I I Q D N N G T I I P F R K H T V R G E R S Y S W G M A V N V Y S T S I T Q E T M S R H D I I A W V N D I V S L N Y T K V E Q L C S G A A Y C Q F M D M L F P G C I S L K K V K F Q A K L E H E Y I H N F K L L Q A S F K R M N V D K V I P V E K L V K G R F Q D N L D F I Q W F K K F Y D A N Y D G K E Y D P V E A R Q G Q D A I P P P D P G E Q I F N L P K K S H H A N S P T A G A A K S S P A A K P G S T P S R P S S A K R A S S S G S A S K S D K D L E T Q V I Q L N E Q V H S L K L A L E G V E K E R D F Y F G K L R E I E L L C Q E H G Q E N D D L V Q R L M D I L Y A S E E H E G H T E E P E A E E Q A H E Q Q P P Q Q E E Y
- Accession
- Q15555
- Predicted molecular weight
- 39.2 kDa
- Amino acids
- 1 to 327
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113592 was purified using conventional chromatography.
General info
- Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).
- Sequence similarities
Belongs to the MAPRE family.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target EB2 protein also known as 'ambien432' or 'kt52' is an important component within cellular mechanics. It has a mass of approximately 55 kDa and expresses in various tissues including the brain and liver. EB2 localizes to the cytoplasm and nucleus where it interacts with different molecular constituents. Researchers find it involved in regulating protein-protein interactions influencing cellular signaling dynamics and further biological processes.
- Biological function summary
The EB2 protein regulates transcriptional activities and modulates gene expression. It integrates into a well-defined complex known as the EB2-NIW complex playing an important role in maintaining cellular homeostasis. This protein frequently acts as a modulator of metabolic pathways having direct implications in critical cellular functions. The interactions within the complex suggest it influences cellular responses to external stimuli adjusting transcriptional output.
- Pathways
The EB2 protein participates actively in two major biological pathways: the MAPK signaling pathway and the PI3K/AKT pathway. Within these pathways EB2 contributes by modulating downstream signaling events. It maintains functional interactions with other proteins such as Ras and Akt ensuring proper signal transduction. These interactions signify EB2's importance in mediating responses that control cell survival and proliferation.
- Associated diseases and disorders
Research has linked EB2 protein activity to oncogenic processes and neurodegenerative diseases. Studies indicate EB2 overexpression in certain cancers where it influences cell cycle regulation through interactions with proteins like p53. In neurodegenerative conditions EB2's regulatory role may impact cellular decay mechanisms with relationships observed with proteins involved in neuronal degradation. Researchers continue to investigate its potential as a therapeutic target for these diseases.
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1 product image
SDS-PAGE - Recombinant Human EB2 protein (ab113592)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
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