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AB113592

Recombinant Human EB2 protein

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Recombinant Human EB2 protein is a Human protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

RP1, MAPRE2, Microtubule-associated protein RP/EB family member 2, APC-binding protein EB2, End-binding protein 2, EB2

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SDS-PAGE - Recombinant Human EB2 protein (AB113592)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EB2 protein (AB113592)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q15555

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPGPTQTLSPNGENNNDIIQDNNGTIIPFRKHTVRGERSYSWGMAVNVYSTSITQETMSRHDIIAWVNDIVSLNYTKVEQLCSGAAYCQFMDMLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKVIPVEKLVKGRFQDNLDFIQWFKKFYDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKSSPAAKPGSTPSRPSSAKRASSSGSASKSDKDLETQVIQLNEQVHSLKLALEGVEKERDFYFGKLREIELLCQEHGQENDDLVQRLMDILYASEEHEGHTEEPEAEEQAHEQQPPQQEEY","proteinLength":null,"predictedMolecularWeight":"39.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15555","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target EB2 protein also known as 'ambien432' or 'kt52' is an important component within cellular mechanics. It has a mass of approximately 55 kDa and expresses in various tissues including the brain and liver. EB2 localizes to the cytoplasm and nucleus where it interacts with different molecular constituents. Researchers find it involved in regulating protein-protein interactions influencing cellular signaling dynamics and further biological processes.
Biological function summary

The EB2 protein regulates transcriptional activities and modulates gene expression. It integrates into a well-defined complex known as the EB2-NIW complex playing an important role in maintaining cellular homeostasis. This protein frequently acts as a modulator of metabolic pathways having direct implications in critical cellular functions. The interactions within the complex suggest it influences cellular responses to external stimuli adjusting transcriptional output.

Pathways

The EB2 protein participates actively in two major biological pathways: the MAPK signaling pathway and the PI3K/AKT pathway. Within these pathways EB2 contributes by modulating downstream signaling events. It maintains functional interactions with other proteins such as Ras and Akt ensuring proper signal transduction. These interactions signify EB2's importance in mediating responses that control cell survival and proliferation.

Research has linked EB2 protein activity to oncogenic processes and neurodegenerative diseases. Studies indicate EB2 overexpression in certain cancers where it influences cell cycle regulation through interactions with proteins like p53. In neurodegenerative conditions EB2's regulatory role may impact cellular decay mechanisms with relationships observed with proteins involved in neuronal degradation. Researchers continue to investigate its potential as a therapeutic target for these diseases.
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Specifications

Form

Liquid

Additional notes

ab113592 was purified using conventional chromatography.

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General info

Function

May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).

Sequence similarities

Belongs to the MAPRE family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).
See full target information MAPRE2
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