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AB101197

Recombinant Human EB3 protein

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Recombinant Human EB3 protein is a Human Full Length protein, in the 1 to 281 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Microtubule-associated protein RP/EB family member 3, EB1 protein family member 3, End-binding protein 3, RP3, EBF3, EB3, MAPRE3

1 Images
SDS-PAGE - Recombinant Human EB3 protein (AB101197)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EB3 protein (AB101197)

15% SDS-PAGE showing ab101197 at approximately 34.1kDa (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9UPY8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY","proteinLength":"Full Length","predictedMolecularWeight":"34.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":281,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UPY8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EB3 also known as End-binding protein 3 is a microtubule-associated protein with a molecular weight of approximately 32 kDa. This protein belongs to the EB family of proteins which includes EB1 and EB2. EB3 facilitates microtubule dynamics by binding to the plus ends of microtubules and playing a role in their stabilization and elongation. Researchers have observed its expression in various tissues with a notable presence in neuronal tissues where it supports complex cellular activities.
Biological function summary

EB3 interacts with other end-binding proteins as part of the plus-end tracking complex often abbreviated as +TIPs. This complex modulates the behavior of microtubules essential for processes like mitosis and cell migration. EB3’s interaction within this complex stabilizes microtubules at the growing edge important for neuronal development and synaptic function. Additionally EB3 helps in intracellular signaling through direct interaction with other microtubule-associated proteins thereby contributing to cellular structural integrity.

Pathways

EB3 plays an integral role in the Wnt signaling pathway and the microtubule polymerization regulation pathway. These pathways are important for normal cell division and signaling. In the Wnt signaling pathway EB3 interacts with proteins like APC essential for the pathway’s downstream signaling events that influence cell polarity and differentiation. Moreover its involvement in the polymerization regulation pathway helps in facilitating effective cargo transport along microtubules.

EB3 has been linked to neurodegenerative diseases such as Alzheimer’s disease and certain types of cancer. Abnormalities in EB3 expression and function can lead to disruptions in microtubule stability contributing to the pathogenesis of these conditions. EB3's connection with tau protein further highlights its role in Alzheimer’s where tau aggregation negatively affects microtubule function. In cancer alterations in EB3 expression influence tumor progression by impacting cell motility and invasiveness with implications for metastasis development.
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Specifications

Form

Liquid

Additional notes

ab101197 is purified using conventional chromatography techniques.

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General info

Function

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed : 19255245, PubMed : 28814570). Promotes microtubule growth (PubMed : 19255245, PubMed : 28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed : 19255245, PubMed : 28814570). Also acts as a regulator of minus-end microtubule organization : interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed : 28814570).

Sequence similarities

Belongs to the MAPRE family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed : 19255245, PubMed : 28814570). Promotes microtubule growth (PubMed : 19255245, PubMed : 28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed : 19255245, PubMed : 28814570). Also acts as a regulator of minus-end microtubule organization : interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed : 28814570).
See full target information MAPRE3
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