JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB95350

Recombinant Human EBP1 protein (His tag C-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human EBP1 protein (His tag C-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

EBP1, PA2G4, Proliferation-associated protein 2G4, Cell cycle protein p38-2G4 homolog, ErbB3-binding protein 1, hG4-1

2 Images
Western blot - Recombinant Human EBP1 protein (His tag C-Terminus) (AB95350)
  • WB

Unknown

Western blot - Recombinant Human EBP1 protein (His tag C-Terminus) (AB95350)

All lanes:

Anti-EBP1 antibody (<a href='/en-us/products/unavailable/ebp1-antibody-ab33613'>ab33613</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human EBP1 protein (His tag C-Terminus) (ab95350) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 4min

SDS-PAGE - Recombinant Human EBP1 protein (AB95350)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EBP1 protein (AB95350)

15% SDS-PAGE showing ab95350 at approximately 45 kDa (3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

Q9UQ80

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab95350 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/ebp1-antibody-ab33613'>ab33613</a>.</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGDLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UQ80","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EBP1 protein also known as ErbB3-binding protein 1 plays an important role in cellular mechanisms. With a mass of about 39 kDa EBP1 is widely expressed in various tissues including the heart liver and brain. The protein acts as an RNA-binding protein and it has demonstrated influence in the regulation of ribosomal RNA synthesis and processing. EBP1 also interacts with multiple other proteins which aid in its regulatory roles in the cells.
Biological function summary

EBP1 functions as a part of the cellular machinery involved in growth and differentiation. It associates with the eukaryotic translation initiation factor 4G (eIF4G) as part of a complex which modulates the translation of certain messenger RNAs. Furthermore EBP1 can function as a transcriptional co-repressor in the nucleus where it influences gene expression by interacting with DNA. Its activity in these roles highlights its important function in cell cycle regulation and cellular proliferation.

Pathways

Many signaling mechanisms engage EBP1. The PI3K/AKT pathway sees participation from EBP1 where it downstream affects cell survival and growth. EBP1 also interacts with the NF-κB pathway contributing to the regulation of immune and stress responses. In these pathways interactions with key proteins like AKT and NF-κB p65 emphasize its role in integrating signaling cues for responses to environmental stimuli.

EBP1 has shown associations with cancer and neurodegenerative diseases. Its connection with cancer is evident as EBP1 influences cell proliferation and can affect tumor suppressor pathways sometimes interacting with proteins like HER2. In neurodegenerative diseases deregulation of EBP1 function has been seen in conditions such as Alzheimer's potentially interacting with proteins involved in tau phosphorylation. Both examples demonstrate the biological significance of EBP1 in maintaining normal physiological function and its contributions to pathology when dysregulated.
style
left-column

Specifications

Form

Liquid

Additional notes

ab95350 is purified using conventional chromatography techniques.

style
left-column

General info

Function

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

Sequence similarities

Belongs to the peptidase M24 family.

Post-translational modifications

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.. In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).
See full target information PA2G4
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB180602

Anti-EBP1 antibody [EPR14569(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody [EPR14569(B)] (AB180602)

  • 0
  • 0
Secondary Antibodies

AB150093

Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 488)

Alexa Fluor® - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 488) (AB150093)

  • 0
  • 0
Proteins & Peptides

AB53869

Recombinant Human 14-3-3 gamma/YWHAG protein (Tag Free)

SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (AB53869)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com