Recombinant Human EBV Induced Gene 2/EBI2 protein is a Human Full Length protein, in the 1 to 361 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M D I Q M A N N F T P P S A T P Q G N D C D L Y A H H S T A R I V M P L H Y S L V F I I G L V G N L L A L V V I V Q N R K K I N S T T L Y S T N L V I S D I L F T T A L P T R I A Y Y A M G F D W R I G D A L C R I T A L V F Y I N T Y A G V N F M T C L S I D R F I A V V H P L R Y N K I K R I E H A K G V C I F V W I L V F A Q T L P L L I N P M S K Q E A E R I T C M E Y P N F E E T K S L P W I L L G A C F I G Y V L P L I I I L I C Y S Q I C C K L F R T A K Q N P L T E K S G V N K K A L N T I I L I I V V F V L C F T P Y H V A I I Q H M I K K L R F S N F L E C S Q R H S F Q I S L H F T V C L M N F N C C M D P F I Y F F A C K G Y K R K V M R M L K R Q V S V S I S S A V K S A P E E N S R E M T E T Q M M I H S K S S N G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity). Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (PubMed:21796212, PubMed:22875855, PubMed:22930711). Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity). Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity). Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity). Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (PubMed:22913878). Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity). Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity). Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels (By similarity). Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (PubMed:25297897). Promotes osteoclast precursor migration to bone surfaces (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha (PubMed:21673108, PubMed:25297897). Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity).
Alternative names
EBI2, GPR183, G-protein coupled receptor 183, Epstein-Barr virus-induced G-protein coupled receptor 2, EBV-induced G-protein coupled receptor 2, hEBI2
Recommended products
Recombinant Human EBV Induced Gene 2/EBI2 protein is a Human Full Length protein, in the 1 to 361 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P32249-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M D I Q M A N N F T P P S A T P Q G N D C D L Y A H H S T A R I V M P L H Y S L V F I I G L V G N L L A L V V I V Q N R K K I N S T T L Y S T N L V I S D I L F T T A L P T R I A Y Y A M G F D W R I G D A L C R I T A L V F Y I N T Y A G V N F M T C L S I D R F I A V V H P L R Y N K I K R I E H A K G V C I F V W I L V F A Q T L P L L I N P M S K Q E A E R I T C M E Y P N F E E T K S L P W I L L G A C F I G Y V L P L I I I L I C Y S Q I C C K L F R T A K Q N P L T E K S G V N K K A L N T I I L I I V V F V L C F T P Y H V A I I Q H M I K K L R F S N F L E C S Q R H S F Q I S L H F T V C L M N F N C C M D P F I Y F F A C K G Y K R K V M R M L K R Q V S V S I S S A V K S A P E E N S R E M T E T Q M M I H S K S S N G K
- Accession
- P32249
- Protein length
- Full Length
- Amino acids
- 1 to 361
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity). Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (PubMed:21796212, PubMed:22875855, PubMed:22930711). Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity). Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity). Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity). Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (PubMed:22913878). Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity). Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity). Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels (By similarity). Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (PubMed:25297897). Promotes osteoclast precursor migration to bone surfaces (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha (PubMed:21673108, PubMed:25297897). Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity).
- Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EBV Induced Gene 2 (EBI2) also known as GPR183 is a G protein-coupled receptor with a mass of approximately 41 kDa. It is prominently expressed in immune cells such as B cells and T cells. EBI2 is activated by oxysterols which are oxidized derivatives of cholesterol and triggers a signaling cascade that influences cell positioning and migration. The receptor plays an important role in guiding B cell migration during immune responses especially within lymphoid tissues.
- Biological function summary
EBI2 supports the positioning of immune cells within secondary lymphoid organs by detecting specific chemokine gradients. It does not form part of larger complexes but instead functions independently to interpret chemotactic signals. EBI2 is important during B cell maturation orchestrating the cellular movement that is essential for executing adaptive immune responses effectively.
- Pathways
EBI2 integrates into the oxysterol signaling pathway which directly impacts immune cell dynamics. Through its effects on B cell migration its function connects to the chemokine signaling pathway. EBI2 interacts with proteins like CXCR5 enabling the directed movement of B cells towards follicular dendritic cells playing a role in ensuring effective humoral immunity.
- Associated diseases and disorders
Irregular EBI2 expression correlates with autoimmune conditions such as rheumatoid arthritis and lupus. Aberrant signaling through EBI2 can contribute to improper immune cell localization which is a characteristic of these disorders. The receptor's interplay with CXCR5 is particularly significant in the context of rheumatoid arthritis as both are implicated in the dysregulation of immune cell homing leading to inflammation.
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1 product image
SDS-PAGE - Recombinant Human EBV Induced Gene 2/EBI2 protein (ab158331)
ab158331 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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