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AB276334

Recombinant Human ECE1 protein (His tag)

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Recombinant Human ECE1 protein (His tag) is a Human Fragment protein, in the 90 to 770 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Endothelin-converting enzyme 1, ECE-1, ECE1

1 Images
SDS-PAGE - Recombinant Human ECE1 protein (His tag) (AB276334)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ECE1 protein (His tag) (AB276334)

SDS-PAGE analysis of ab276334

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P42892

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"QYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW","proteinLength":"Fragment","predictedMolecularWeight":"80 kDa","actualMolecularWeight":null,"aminoAcidEnd":770,"aminoAcidStart":90,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P42892","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ECE1 gene encodes for endothelin-converting enzyme 1 also referred to as ECE-1. This enzyme weighs approximately 120 kDa and functions mechanically to convert big endothelins into active endothelins. ECE-1 is present in many tissues with higher expression in the endothelium kidneys and lungs. Through its metalloprotease activity ECE-1 plays a central role in regulating endothelin peptide activation which is important for vasoconstriction and other physiological processes.
Biological function summary

ECE-1 takes part in maintaining vascular homeostasis and blood pressure regulation. It does not function as part of a complex but exerts its effects through the cleavage of specific precursor proteins such as big endothelins. By controlling the conversion of these precursors to active peptidic forms ECE-1 directly influences the vascular tone and endothelial function.

Pathways

ECE-1 impacts the endothelin signaling pathway significantly where it plays a role in converting big endothelin-1 to a potent vasoconstrictor endothelin-1. This pathway involves other proteins like endothelin receptors which mediate the physiological effects of endothelins on smooth muscles and vascular tissues. Additionally ECE-1 interacts with the renin-angiotensin-aldosterone system (RAAS) linking it to blood pressure modulation and sodium balance.

ECE-1 is associated with conditions such as hypertension and pulmonary arterial hypertension (PAH). Elevated activity or expression levels can contribute to increased vascular resistance leading to heightened blood pressure. Through its role in endothelin signaling ECE-1 also links to proteins like endothelin-1 which are implicated in the pathophysiology of these cardiovascular disorders.
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General info

Function

Converts big endothelin-1 to endothelin-1.

Sequence similarities

Belongs to the peptidase M13 family.

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Product protocols

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Target data

Converts big endothelin-1 to endothelin-1.
See full target information Endothelin-converting enzyme 1
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