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AB276228

Recombinant Human ECE2 protein (His tag)

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Recombinant Human ECE2 protein (His tag) is a Human Fragment protein, in the 200 to 883 aa range, expressed in HEK 293 cells, with >93%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

EEF1AKMT4-ECE2 readthrough transcript protein, EEF1AKMT4-ECE2

1 Images
SDS-PAGE - Recombinant Human ECE2 protein (His tag) (AB276228)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ECE2 protein (His tag) (AB276228)

SDS-PAGE analysis of ab276228

Key facts

Purity

>93% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P0DPD8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"GVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW","proteinLength":"Fragment","predictedMolecularWeight":"80.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":883,"aminoAcidStart":200,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P0DPD8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECE2 also known as Endothelin-Converting Enzyme 2 is a metalloprotease with a molecular mass of approximately 85 kDa. It plays a role in converting inactive big endothelins into active endothelins which are potent vasoactive peptides. You find ECE2 expressed in various tissues including the brain heart and lungs. The protein localizes mainly to the cell membrane and endosomal compartments where it performs its enzymatic functions.
Biological function summary

ECE2 has a significant role in the regulation of vascular tone and blood pressure. It is an integral part of the endothelin system not forming a complex but working along with endothelin receptors to modulate vasoconstriction and vasodilation. Its activity helps maintain cardiovascular homeostasis. The enzyme processes peptides that influence neuronal signaling suggesting a role in neural function as well.

Pathways

ECE2 features prominently in the endothelin signaling pathway and the broader cardiovascular regulatory pathway. It processes the precursor proteins big endothelins which then activate endothelin receptors (EDNRA EDNRB). These receptors further engage with G-proteins to promote downstream signaling that regulates vascular constriction and blood pressure. ECE2 activity is important for ensuring the balance of these signaling cascades particularly in cardiovascular and neural contexts.

ECE2 plays a part in the pathophysiology of hypertension and Alzheimer's disease. Altered ECE2 activity influences endothelin levels contributing to blood pressure dysregulation and hypertension. In Alzheimer's disease ECE2 works with proteins like beta-amyloid peptide where dysregulation may lead to neurotoxicity and plaque formation. These connections make ECE2 a potential therapeutic target in disease intervention.
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Specifications

Form

Lyophilized

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General info

Function

Converts big endothelin-1 to endothelin-1. May also have methyltransferase activity (By similarity). May play a role in amyloid-beta processing (By similarity).

Sequence similarities

In the N-terminal section; belongs to the methyltransferase superfamily.. In the C-terminal section; belongs to the peptidase M13 family.

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Product protocols

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Target data

Converts big endothelin-1 to endothelin-1. May also have methyltransferase activity (By similarity). May play a role in amyloid-beta processing (By similarity).
See full target information EEF1AKMT4-ECE2
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Product promise

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