Recombinant Human ECE2 protein (His tag) is a Human Fragment protein, in the 200 to 883 aa range, expressed in HEK 293, with >93% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >93% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
G V Q Y H R D P S H S T C L T E A C I R V A G K I L E S L D R G V S P C E D F Y Q F S C G G W I R R N P L P D G R S R W N T F N S L W D Q N Q A I L K H L L E N T T F N S S S E A E Q K T Q R F Y L S C L Q V E R I E E L G A Q P L R D L I E K I G G W N I T G P W D Q D N F M E V L K A V A G T Y R A T P F F T V Y I S A D S K S S N S N V I Q V D Q S G L F L P S R D Y Y L N R T A N E K V L T A Y L D Y M E E L G M L L G G R P T S T R E Q M Q Q V L E L E I Q L A N I T V P Q D Q R R D E E K I Y H K M S I S E L Q A L A P S M D W L E F L S F L L S P L E L S D S E P V V V Y G M D Y L Q Q V S E L I N R T E P S I L N N Y L I W N L V Q K T T S S L D R R F E S A Q E K L L E T L Y G T K K S C V P R W Q T C I S N T D D A L G F A L G S L F V K A T F D R Q S K E I A E G M I S E I R T A F E E A L G Q L V W M D E K T R Q A A K E K A D A I Y D M I G F P D F I L E P K E L D D V Y D G Y E I S E D S F F Q N M L N L Y N F S A K V M A D Q L R K P P S R D Q W S M T P Q T V N A Y Y L P T K N E I V F P A G I L Q A P F Y A R N H P K A L N F G G I G V V M G H E L T H A F D D Q G R E Y D K E G N L R P W W Q N E S L A A F R N H T A C M E E Q Y N Q Y Q V N G E R L N G R Q T L G E N I A D N G G L K A A Y N A Y K A W L R K H G E E Q Q L P A V G L T N H Q L F F V G F A Q V W C S V R T P E S S H E G L V T D P H S P A R F R V L G T L S N S R D F L R H F G C P V G S P M N P G Q L C E V W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Converts big endothelin-1 to endothelin-1. May also have methyltransferase activity (By similarity). May play a role in amyloid-beta processing (By similarity).
Alternative names
EEF1AKMT4-ECE2 readthrough transcript protein, EEF1AKMT4-ECE2
Recombinant Human ECE2 protein (His tag) is a Human Fragment protein, in the 200 to 883 aa range, expressed in HEK 293, with >93% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >93% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P0DPD8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- G V Q Y H R D P S H S T C L T E A C I R V A G K I L E S L D R G V S P C E D F Y Q F S C G G W I R R N P L P D G R S R W N T F N S L W D Q N Q A I L K H L L E N T T F N S S S E A E Q K T Q R F Y L S C L Q V E R I E E L G A Q P L R D L I E K I G G W N I T G P W D Q D N F M E V L K A V A G T Y R A T P F F T V Y I S A D S K S S N S N V I Q V D Q S G L F L P S R D Y Y L N R T A N E K V L T A Y L D Y M E E L G M L L G G R P T S T R E Q M Q Q V L E L E I Q L A N I T V P Q D Q R R D E E K I Y H K M S I S E L Q A L A P S M D W L E F L S F L L S P L E L S D S E P V V V Y G M D Y L Q Q V S E L I N R T E P S I L N N Y L I W N L V Q K T T S S L D R R F E S A Q E K L L E T L Y G T K K S C V P R W Q T C I S N T D D A L G F A L G S L F V K A T F D R Q S K E I A E G M I S E I R T A F E E A L G Q L V W M D E K T R Q A A K E K A D A I Y D M I G F P D F I L E P K E L D D V Y D G Y E I S E D S F F Q N M L N L Y N F S A K V M A D Q L R K P P S R D Q W S M T P Q T V N A Y Y L P T K N E I V F P A G I L Q A P F Y A R N H P K A L N F G G I G V V M G H E L T H A F D D Q G R E Y D K E G N L R P W W Q N E S L A A F R N H T A C M E E Q Y N Q Y Q V N G E R L N G R Q T L G E N I A D N G G L K A A Y N A Y K A W L R K H G E E Q Q L P A V G L T N H Q L F F V G F A Q V W C S V R T P E S S H E G L V T D P H S P A R F R V L G T L S N S R D F L R H F G C P V G S P M N P G Q L C E V W
- Accession
- P0DPD8
- Protein length
- Fragment
- Predicted molecular weight
- 80.2 kDa
- Amino acids
- 200 to 883
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Converts big endothelin-1 to endothelin-1. May also have methyltransferase activity (By similarity). May play a role in amyloid-beta processing (By similarity).
- Sequence similarities
In the N-terminal section; belongs to the methyltransferase superfamily.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ECE2 also known as Endothelin-Converting Enzyme 2 is a metalloprotease with a molecular mass of approximately 85 kDa. It plays a role in converting inactive big endothelins into active endothelins which are potent vasoactive peptides. You find ECE2 expressed in various tissues including the brain heart and lungs. The protein localizes mainly to the cell membrane and endosomal compartments where it performs its enzymatic functions.
- Biological function summary
ECE2 has a significant role in the regulation of vascular tone and blood pressure. It is an integral part of the endothelin system not forming a complex but working along with endothelin receptors to modulate vasoconstriction and vasodilation. Its activity helps maintain cardiovascular homeostasis. The enzyme processes peptides that influence neuronal signaling suggesting a role in neural function as well.
- Pathways
ECE2 features prominently in the endothelin signaling pathway and the broader cardiovascular regulatory pathway. It processes the precursor proteins big endothelins which then activate endothelin receptors (EDNRA EDNRB). These receptors further engage with G-proteins to promote downstream signaling that regulates vascular constriction and blood pressure. ECE2 activity is important for ensuring the balance of these signaling cascades particularly in cardiovascular and neural contexts.
- Associated diseases and disorders
ECE2 plays a part in the pathophysiology of hypertension and Alzheimer's disease. Altered ECE2 activity influences endothelin levels contributing to blood pressure dysregulation and hypertension. In Alzheimer's disease ECE2 works with proteins like beta-amyloid peptide where dysregulation may lead to neurotoxicity and plaque formation. These connections make ECE2 a potential therapeutic target in disease intervention.
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1 product image
SDS-PAGE - Recombinant Human ECE2 protein (His tag) (ab276228)
SDS-PAGE analysis of ab276228
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