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AB123187

Recombinant Human ECHDC1 protein (His tag N-Terminus)

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Recombinant Human ECHDC1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 307 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Ethylmalonyl-CoA decarboxylase, Enoyl-CoA hydratase domain-containing protein 1, Methylmalonyl-CoA decarboxylase, MMCD, ECHDC1

1 Images
SDS-PAGE - Recombinant Human ECHDC1 protein (His tag N-Terminus) (AB123187)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ECHDC1 protein (His tag N-Terminus) (AB123187)

15% SDS-PAGE showing ab123187 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9NTX5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMALKQEMAKSLLKTASLSGRTKLLHQTGLSLYSTSHGFYEEEVKKTLQQFPGGSIDLQKEDNGIGILTLNNPSRMNAFSGVMMLQLLEKVIELENWTEGKGLIVRGAKNTFSSGSDLNAVKSLGTPEDGMAVCMFMQNTLTRFMRLPLISVALVQGWALGGGAEFTTACDFRLMTPESKIRFVHKEMGIIPSWGGTTRLVEIIGSRQALKVLSGALKLDSKNALNIGMVEEVLQSSDETKSLEEAQEWLKQFIQGPPEVIRALKKSVCSGRELYLEEALQNERDLLGTVWGGPANLEAIAKKGKFNKGGAEFTTACDFRLMTPESKIRFVHKEMGIIPSWGGTTRLVEIIGSRQALKVLSGALKLDSKNALNIGMVEEVLQSSDETKSLEEAQEWLKQFIQGPPEVIRALKKSVCSGRELYLEEALQNERDLLGTVWGGPANLEAIAKKGKFNK","proteinLength":"Full Length","predictedMolecularWeight":"35.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":307,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NTX5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECHDC1 also known as 'enoyl-CoA hydratase domain-containing protein 1' plays a role in fatty acid metabolism through its enzymatic activity. It weighs around 39 kDa and can be found in various tissues but it has higher expression levels in the heart and muscle tissues. This protein participates in metabolic processes by catalyzing the hydration of enoyl-CoA during the beta-oxidation of fatty acids.
Biological function summary

EVT-888 (another name for ECHDC1 used in some studies) acts as a component that affects metabolic pathways by mediating reactions associated with energy production. Though it is not actively considered formally as a part of large enzyme complexes ECHDC1 indirectly relates to mitochondrial function and energy regulation. It influences long-chain fatty acid breakdown assisting cells to maintain energy homeostasis.

Pathways

ECHDC1 influences key metabolic pathways like the beta-oxidation pathway of fatty acids and the mitochondrial respiration chain. This protein interacts closely with other proteins such as ACADVL (very long-chain specific acyl-CoA dehydrogenase) and HADHA (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha) playing a central role in energy conversion and mitochondrial efficiency.

Alterations in ECHDC1 expression or function may associate with metabolic syndromes and cardiomyopathies due to its involvement in fatty acid metabolism and energy production. Disrupted interactions with metabolic proteins like HADHA and ACADVL can result in energy production deficits linking ECHDC1 to inherited metabolic disorders related to mitochondrial anomalies.
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Specifications

Form

Liquid

Additional notes

ab123187 is purified using conventional chromatography techniques.

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General info

Function

Decarboxylates ethylmalonyl-CoA, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading (PubMed : 22016388). Acts preferentially on (S)-ethylmalonyl-CoA but has also some activity on the (R)-isomer (By similarity). Also has methylmalonyl-CoA decarboxylase activity at lower level (By similarity).

Sequence similarities

Belongs to the enoyl-CoA hydratase/isomerase family.

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Product protocols

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Target data

Decarboxylates ethylmalonyl-CoA, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading (PubMed : 22016388). Acts preferentially on (S)-ethylmalonyl-CoA but has also some activity on the (R)-isomer (By similarity). Also has methylmalonyl-CoA decarboxylase activity at lower level (By similarity).
See full target information ECHDC1
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