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AB134621

Recombinant Human ECSIT protein (His tag N-Terminus)

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Recombinant Human ECSIT protein (His tag N-Terminus) is a Human Fragment protein, in the 19 to 217 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Protein SITPEC, ECSIT

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SDS-PAGE - Recombinant Human ECSIT protein (His tag N-Terminus) (AB134621)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ECSIT protein (His tag N-Terminus) (AB134621)

15% SDS-PAGE analysis of 3 µg ab134621.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9BQ95

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSGTCGAALTGTSISQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS","proteinLength":"Fragment","predictedMolecularWeight":"24.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":217,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BQ95","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ECSIT also known as Evolutionarily Conserved Signaling Intermediate in Toll pathways is a mitochondrial protein involved in various cellular processes. It has a molecular mass of approximately 52 kDa and is prominently expressed in tissues with high metabolic activity such as the heart and skeletal muscles. ECSIT interacts with the mitochondrial membrane and is integral for mitochondrial function and biogenesis. Understanding the expression and function of ECSIT can provide insight into its role in cellular energy homeostasis.
Biological function summary

ECSIT influences several important cellular processes through its participation in the assembly of the mitochondrial complex I. It is part of a protein complex that includes NDUFAF1 and acts as a scaffold protein facilitating the proper assembly of complex I. ECSIT involvement is important for the maintenance of cellular respiration and energy production. Its role in complex I assembly highlights its importance in regulating mitochondrial activity and function.

Pathways

ECSIT acts in both mitochondrial biogenesis and the toll-like receptor (TLR) signaling pathway. In the TLR pathway ECSIT mediates interactions between TRAF6 and TAK1 contributing to the regulation of immune responses. It is an essential component linking mitochondrial function with immune signaling demonstrating its multi-functional capability. These pathways highlight the intricate network where ECSIT collaborates with proteins like TRAF6 and TAK1 to ensure proper immune response and energy metabolism.

ECSIT has associations with leigh syndrome and aggressive lung cancer. Its dysfunction in mitochondrial biogenesis can contribute to Leigh syndrome a severe neurological disorder. In lung cancer ECSIT involvement in TLR signaling affects cell proliferation and survival making it a protein of interest in cancer research. ECSIT's role in these conditions can involve interactions with mitochondrial proteins and signaling molecules like TRAF6 further illustrating its impact on human health.
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Specifications

Form

Liquid

Additional notes

ab134621 is purified using conventional chromatography techniques.

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General info

Function

Adapter protein that plays a role in different signaling pathways including TLRs and IL-1 pathways or innate antiviral induction signaling. Plays a role in the activation of NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7 to activate TAK1/MAP3K7 leading to activation of IKKs (PubMed : 25355951, PubMed : 31281713). Once ubiquitinated, interacts with the dissociated RELA and NFKB1 proteins and translocates to the nucleus where it induces NF-kappa-B-dependent gene expression (PubMed : 25355951). Plays a role in innate antiviral immune response by bridging the pattern recognition receptors RIGI and MDA5/IFIT1 to the MAVS complex at the mitochondrion (PubMed : 25228397). Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).. As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.

Sequence similarities

Belongs to the ECSIT family.

Post-translational modifications

Ubiquitinated on Lys-372; leading to translocation in the nucleus together with RELA and NFKB1 and expression of NF-kappa-B-dependent genes.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Adapter protein that plays a role in different signaling pathways including TLRs and IL-1 pathways or innate antiviral induction signaling. Plays a role in the activation of NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7 to activate TAK1/MAP3K7 leading to activation of IKKs (PubMed : 25355951, PubMed : 31281713). Once ubiquitinated, interacts with the dissociated RELA and NFKB1 proteins and translocates to the nucleus where it induces NF-kappa-B-dependent gene expression (PubMed : 25355951). Plays a role in innate antiviral immune response by bridging the pattern recognition receptors RIGI and MDA5/IFIT1 to the MAVS complex at the mitochondrion (PubMed : 25228397). Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).. As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
See full target information ECSIT
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AB21288

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Primary Antibodies

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