JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB132637

Recombinant Human EDC3 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human EDC3 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 508 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

LSM16, YJDC, YJEFN2, PP844, EDC3, Enhancer of mRNA-decapping protein 3, LSM16 homolog, YjeF N-terminal domain-containing protein 2, YjeF domain-containing protein 1, YjeF_N2, hYjeF_N2

1 Images
SDS-PAGE - Recombinant Human EDC3 protein (GST tag N-Terminus) (AB132637)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EDC3 protein (GST tag N-Terminus) (AB132637)

12.5% SDS-PAGE analysis of ab132637 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

Q96F86

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB168851

Anti-EDC3 antibody

Immunoprecipitation - Anti-EDC3 antibody (AB168851)

  • 0
  • 0
Primary Antibodies

AB183709

Anti-Dcp1a antibody [EPR13822]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (AB183709)

  • 0
  • 0
Primary Antibodies

AB46803

Anti-MAG/GMA antibody [EP971Y]

RabMAb|Recombinant

Western blot - Anti-MAG/GMA antibody [EP971Y] (AB46803)

  • 1
  • 3
Primary Antibodies

AB72408

Anti-EDC4 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody (AB72408)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MATDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELKILEIPGPGDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSAPQNIPKRTDVKSQDVAVSPQQQQCSKSYVDRHMESLSQSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNLALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA","proteinLength":"Full Length","predictedMolecularWeight":"81.62 kDa","actualMolecularWeight":null,"aminoAcidEnd":508,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96F86","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EDC3 protein also known as Enhancer of mRNA Decapping 3 serves an essential role in mRNA processing and turnover. It functions mechanically within the RNA decay pathway aiding in the removal of the cap structure from mRNA a step important for mRNA degradation. EDC3 with a molecular mass of approximately 59 kDa is expressed in various tissues notably in brain and testis among others. It interacts with multiple RNA-binding proteins that support the assembly of mRNA decay machinery.
Biological function summary

EDC3 supports mRNA regulation by participating in mRNA decapping a regulatory process essential for controlling gene expression. EDC3 is a critical component of the decapping complex interacting with proteins like DCP1 and DCP2 facilitating efficient decapping of mRNA. The interaction of EDC3 with Pat1 another decapping activator enhances the mRNA decay process and ensures proper turnover of mRNA in the cell.

Pathways

EDC3 plays a part in the 5' to 3' mRNA decay pathway facilitating the removal of the cap structure leading to exonucleolytic degradation of mRNA. This pathway is important in mRNA degradation and turnover ensuring precise regulation of gene expression. EDC3 is related to proteins such as LSM1 and XRN1 which cooperate within the 5' to 3' exonucleolytic decay pathway promoting efficient mRNA degradation.

EDC3 alterations may associate with neurological conditions and cancer. Studies suggest that disruptions in mRNA decay where EDC3 plays a central role can lead to improper gene expression contributing to neuronal dysfunctions in neurological diseases. Also anomalies in decay pathways may link to oncogenic processes potentially pointing to EDC3's involvement in cancer development. EDC3’s interaction with proteins like DCP2 and XRN1 in the context of these disorders reveals the significance of RNA processing in maintaining cellular homeostasis and preventing disease progression.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis.

Sequence similarities

Belongs to the EDC3 family.

style
left-column

Product protocols

style
left-column

Target data

Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis.
See full target information EDC3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com