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AB276680

Recombinant Human EDEM2 protein (His tag)

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Recombinant Human EDEM2 protein (His tag) is a Human Fragment protein, in the 1 to 492 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

C20orf31, C20orf49, UNQ573/PRO1135, EDEM2, ER degradation-enhancing alpha-mannosidase-like protein 2

1 Images
SDS-PAGE - Recombinant Human EDEM2 protein (His tag) (AB276680)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EDEM2 protein (His tag) (AB276680)

SDS-PAGE analysis of ab276680

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9BV94

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFYHAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQDSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKLMAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMRTFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNNGSTFDAVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLK","proteinLength":"Fragment","predictedMolecularWeight":"54 kDa","actualMolecularWeight":null,"aminoAcidEnd":492,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9BV94","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein EDEM2 is an important part of endoplasmic reticulum-associated degradation (ERAD). It also goes by the name ER degradation-enhancing alpha-mannosidase-like protein 2. This protein is about 66 kDa in mass. EDEM2 is mostly expressed in cells within the endoplasmic reticulum where it carries out its main function. The protein helps in recognizing misfolded glycoproteins and it plays a role in the removal of terminal mannose residues which is necessary for processing and degradation.
Biological function summary

EDEM2 helps ensure protein quality control within cells. It works as part of the ERAD system by targeting misfolded glycoproteins for degradation preventing accumulation of defective proteins that could be harmful to cells. EDEM2 does not act alone but instead functions together with other molecular chaperones and lectin-like proteins to identify and process substrates. This cooperation is essential for maintaining cellular homeostasis and avoiding stress responses.

Pathways

EDEM2 links closely with both protein foldings and quality control mechanisms. It directly interacts with ERAD pathways promoting the disassembly of structurally improper glycoproteins. The action of EDEM2 in these pathways connects with other proteins like SEL1L and HRD1 forming a network that handles aberrant proteins.

EDEM2's role in ERAD pathways relates to conditions like cystic fibrosis and certain neurodegenerative diseases. Misfolded proteins are a known feature in these diseases and EDEM2’s function is essential for managing such protein folding errors. Problems in the activity of EDEM2 or related proteins like OS9 can potentially exacerbate these conditions highlighting the importance of EDEM2 in disease management and potential therapeutic approaches.
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Specifications

Form

Lyophilized

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General info

Function

Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner (PubMed : 15537790, PubMed : 25092655). May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2 (PubMed : 25092655). Seems to recognize and bind to exposed hydrophobic regions in target proteins (By similarity).

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner (PubMed : 15537790, PubMed : 25092655). May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2 (PubMed : 25092655). Seems to recognize and bind to exposed hydrophobic regions in target proteins (By similarity).
See full target information EDEM2
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