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AB152354

Recombinant Human EDG2 /LPA-1 protein

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Recombinant Human EDG2 /LPA-1 protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

EDG2, LPA1, LPAR1, Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Lysophosphatidic acid receptor Edg-2

1 Images
SDS-PAGE - Recombinant Human EDG2 /LPA-1 protein (AB152354)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EDG2 /LPA-1 protein (AB152354)

12.5% SDS-PAGE analysis of ab152354 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

Q92633

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

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Sequence info

[{"sequence":"MAAISTSIPVISQPQFTAMNEPQCFYNESIAFFYNRSGKHLATEWNTVSKLVMGLGITVCIFIMLANLLVMVAIYVNRRFHFPIYYLMANLAAADFFAGLAYFYLMFNTGPNTRRLTVSTWLLRQGLIDTSLTASVANLLAIAIERHITVFRMQLHTRMSNRRVVVVIVVIWTMAIVMGAIPSVGWNCICDIENCSNMAPLYSDSYLVFWAIFNLVTFVVMVVLYAHIFGYVRQRTMRMSRHSSGPRRNRDTMMSLLKTVVIVLGAFIICWTPGLVLLLLDVCCPQCDVLAYEKFFLLLAEFNSAMNPIIYSYRDKEMSATFRQILCCQRSENPTGPTEGSDRSASSLNHTILAGVHSNDHSVV","proteinLength":"Full Length","predictedMolecularWeight":"67.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":364,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92633","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EDG2 also known as LPA-1 LPAR1 or lysophosphatidic acid receptor 1 acts as a receptor for LPA (lysophosphatidic acid) a bioactive lipid mediator. This protein has a molecular mass of approximately 41 kDa. It is part of the G protein-coupled receptor (GPCR) family. EDG2 is mainly expressed in organs such as the brain heart and lungs. It plays an important role in cellular signal transduction by binding LPA which leads to various cellular responses.
Biological function summary

EDG2/LPA-1 influences several cellular processes including cell proliferation migration and survival. It does not function as part of a larger complex; instead it activates intracellular signaling cascades upon LPA binding. This activation leads to downstream effects that regulate processes like cytoskeletal reorganization and gene expression. Its influence extends to immune response modulation making it significant in understanding cellular behavior and communication.

Pathways

EDG2/LPA-1 is important in the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K/AKT) signaling pathways. These pathways affect cell growth survival and metabolism showcasing the receptor's importance in cellular regulation. Through these pathways EDG2 interacts with multiple proteins including RAC1 a small GTPase involved in cytoskeletal organization and various kinase enzymes that facilitate signal transduction.

EDG2/LPA-1 has associations with cancer and fibrosis. In cancer the receptor influences tumor growth and metastasis due to its role in cell migration and proliferation. It connects to the matrix metalloproteinases (MMPs) in this context which degrade extracellular matrix components to facilitate cancer cell invasion. Additionally in fibrotic diseases EDG2's influence on fibroblast activity leads to tissue scarring and dysfunctional repair. Understanding these connections helps to explore therapeutic targets meant to modulate EDG2/LPA-1 activity in pathological conditions.
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Specifications

Form

Liquid

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General info

Function

Receptor for lysophosphatidic acid (LPA) (PubMed : 19306925, PubMed : 25025571, PubMed : 26091040, PubMed : 9070858). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels (PubMed : 26091040). Signaling triggers an increase of cytoplasmic Ca(2+) levels (PubMed : 19656035, PubMed : 19733258, PubMed : 26091040). Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate (PubMed : 19306925). Signaling mediates activation of down-stream MAP kinases (By similarity). Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction (PubMed : 26091040). Promotes the activation of Rho and the formation of actin stress fibers (PubMed : 26091040). Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1 (By similarity). Through its function as LPA receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding (PubMed : 18066075, PubMed : 19656035, PubMed : 19733258). Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to LPA (By similarity). Inhibits the intracellular ciliogenesis pathway in response to LPA and through AKT1 activation (PubMed : 31204173). Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain (By similarity).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Post-translational modifications

N-glycosylated.

Subcellular localisation

Endosome

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Product protocols

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Target data

Receptor for lysophosphatidic acid (LPA) (PubMed : 19306925, PubMed : 25025571, PubMed : 26091040, PubMed : 9070858). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels (PubMed : 26091040). Signaling triggers an increase of cytoplasmic Ca(2+) levels (PubMed : 19656035, PubMed : 19733258, PubMed : 26091040). Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate (PubMed : 19306925). Signaling mediates activation of down-stream MAP kinases (By similarity). Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction (PubMed : 26091040). Promotes the activation of Rho and the formation of actin stress fibers (PubMed : 26091040). Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1 (By similarity). Through its function as LPA receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding (PubMed : 18066075, PubMed : 19656035, PubMed : 19733258). Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to LPA (By similarity). Inhibits the intracellular ciliogenesis pathway in response to LPA and through AKT1 activation (PubMed : 31204173). Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain (By similarity).
See full target information LPAR1
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Associated Products

Select an associated product type
Alternative Product
Cell Lines & Lysates

AB94195

EDG2 / LPA-1 overexpression 293T lysate (whole cell)

cell-lysates

edg2-lpa-1-overexpression-293t-lysate-whole-cell-ab94195

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