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AB202211

Recombinant Human EDN3 protein (His tag N-Terminus)

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Recombinant Human EDN3 protein (His tag N-Terminus) is a Human Full Length protein, in the 26 to 238 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Endothelin-3, ET-3, Preproendothelin-3, PPET3, EDN3

1 Images
SDS-PAGE - Recombinant Human EDN3 protein (His tag N-Terminus) (AB202211)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EDN3 protein (His tag N-Terminus) (AB202211)

15% SDS-PAGE analysis of ab202211 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P14138

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Endothelin 3.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVEVKDQQSKQALDLHHPKLMPGSGLALAPSTCPRCLFQEGAP","proteinLength":"Full Length","predictedMolecularWeight":"25.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P14138","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelin-3 (EDN3) is a peptide that plays an important role in various physiological processes. It consists of 21 amino acids and has a molecular mass of around 2.4 kDa. It is expressed in specific tissues like intestinal tract and skin and shows high expression in developing embryos. Alternative names for EDN3 include ET-3 and Preproendothelin-3. The gene encoding EDN3 locates on chromosome 20 and produces this peptide through a well-regulated process.
Biological function summary

EDN3 takes part in cellular signaling that influences cell proliferation and migration. It interacts with other proteins and receptors in the endothelin receptor complex which includes subtypes like EDNRB. This interaction often results in modulation of vascular tone and contributes to the development of neural crest-derived cells. EDN3 activity aims to regulate development through its signaling capabilities directly impacting cell dynamics.

Pathways

EDN3 engages in the endothelin signaling pathway influencing key processes such as vasoconstriction and blood pressure regulation. It interacts with related proteins like EDN1 and EDN2 in these pathways. EDN3 also plays a role in the MAPK signaling pathway where it affects cellular responses to stimuli including growth factors and stress. Its involvement is critical for maintaining homeostasis and proper cellular function.

Abnormalities in EDN3 expression associate with conditions like Hirschsprung's disease and Waardenburg syndrome. Mutation in the EDN3 gene often disrupts normal endothelin signaling leading to disruption in neural crest cell development which connects to Hirschsprung's disease. Moreover the protein can interact with other components such as RET and SOX10 which are key players in the pathway disruptions linked to these disorders. Therefore EDN3 remains significant in understanding the molecular basis of these diseases.
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Specifications

Form

Liquid

Additional notes

ab202211 was purified using conventional chromatography.

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General info

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Sequence similarities

Belongs to the endothelin/sarafotoxin family.

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Product protocols

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Target data

Endothelins are endothelium-derived vasoconstrictor peptides.
See full target information Endothelin-3
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