Recombinant Human EEA1 protein is a Human Fragment protein, in the 1312 to 1411 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Publications
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- Journal of autoimmunity 120:1026332021Implication of a lysosomal antigen in the pathogenesis of lupus erythematosus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaud Wilhelm,Srinivasa Reddy Bonam,Nicolas Schall,Mykolas Bendorius,Anne-Sophie Korganow,Catherine Lumbroso,Sylviane MullerPubMed 33932829
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
K V L E L Q R K L D N T T A A V Q E L G R E N Q S L Q I K H T Q A L N R K W A E D N E V Q N C M A C G K G F S V T V R R H H C R Q C G N I F C A E C S A K N A L T P S S K K P V R V C D A C F N D L Q G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Alternative names
ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
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Recombinant Human EEA1 protein is a Human Fragment protein, in the 1312 to 1411 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q15075-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- K V L E L Q R K L D N T T A A V Q E L G R E N Q S L Q I K H T Q A L N R K W A E D N E V Q N C M A C G K G F S V T V R R H H C R Q C G N I F C A E C S A K N A L T P S S K K P V R V C D A C F N D L Q G
- Accession
- Q15075
- Protein length
- Fragment
- Predicted molecular weight
- 36.63 kDa
- Amino acids
- 1312 to 1411
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
- Subcellular localisation
- Early endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is useful for Antibody Production and Protein Array.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
- Biological function summary
EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.
- Pathways
EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.
- Associated diseases and disorders
EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
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1 product image
SDS-PAGE - Recombinant Human EEA1 protein (ab112379)
ab112379 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue
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