JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB81792

Recombinant Human eEF1A1/EF-Tu protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human eEF1A1/EF-Tu protein is a Human Full Length protein, in the 1 to 462 aa range, expressed in Escherichia coli, suitable for SDS-PAGE.

View Alternative Names

EEF1A, EF1A, LENG7, EEF1A1, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1

1 Images
Western blot - Recombinant Human eEF1A1/EF-Tu protein (AB81792)
  • WB

Project5480****

Western blot - Recombinant Human eEF1A1/EF-Tu protein (AB81792)

All lanes:

Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (<a href='/en-us/products/primary-antibodies/eef1a1eef1a2eef1al3-antibody-ab37969'>ab37969</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human eEF1A1/EF-Tu protein (ab81792) at 0.1 µg

Lane 2:

Human EEF1A2 full length protein (<a href='/en-us/products/unavailable/human-eef1a2-full-length-protein-ab40317'>ab40317</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 50 kDa

false

Exposure time: 3min

Key facts

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P68104

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK","proteinLength":"Full Length","predictedMolecularWeight":"76 kDa","actualMolecularWeight":null,"aminoAcidEnd":462,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P68104","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEF1A1 also known as EF-Tu in prokaryotes functions as an elongation factor in the process of protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the ribosome during the elongation phase of translation. eEF1A1 exhibits a mass of approximately 50 kDa and shows high expression levels in various tissues particularly in the liver and neuronal tissues. It shares functional similarities with EF-G which plays a role in translocation during translation. Moreover eEF1A1 appears relevant in cellular processes beyond translation contributing to its importance across cellular functions.
Biological function summary

EEF1A1 acts as an important component in the machinery of protein synthesis and takes part in the elongation factor complex. Within this complex eEF1A1 interacts with other molecules to ensure the accuracy and efficiency of codon-anticodon pairing on the ribosome. Additionally eEF1A1 associates with actin and participates in the cytoskeleton organization. This multifunctional currency of eEF1A1 suggests its involvement in diverse cellular processes beyond classic translation tasks.

Pathways

The role of eEF1A1 in protein synthesis positions it within the larger context of the mTOR signaling pathway important for cellular growth proliferation and metabolism. Since its function bridges translation initiation and elongation it interacts with proteins like mTOR and S6K1. Within the MAPK pathway eEF1A1 shows interactions influencing cellular survival and apoptosis. These interactions connect eEF1A1 to essential regulatory mechanisms that maintain cellular homeostasis and adaptability.

EEF1A1 exhibits significant involvement in cancer and neurodegenerative diseases. Overexpression or mutation of eEF1A1 frequently associates with various cancers implicating its role in tumor progression and cell proliferation. Interactions with proteins such as p53 link eEF1A1 to pathways that influence tumor suppression and genomic stability. In neurodegenerative conditions like Alzheimer's disease altered eEF1A1 behavior and expression affect neuronal viability and tau protein interaction suggesting its potential contribution to the pathogenesis of these disorders.
style
left-column

Specifications

Form

Liquid

Additional notes

ab81792 is purified by an affinity column in combination with FPLC chromatography. Purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity. 1 unit equals 1 nanogram of purified protein.

style
left-column

General info

Function

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed : 17177976).. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.

Sequence similarities

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

Post-translational modifications

ISGylated.. Phosphorylated by TXK (PubMed:17177976). Phosphorylation by PASK increases translation efficiency (PubMed:17595531). Phosphorylated by ROCK2 (PubMed:26497934). Phosphorylation by TGFBR1 inhibits translation elongation (PubMed:20832312).. Trimethylated at Lys-79 by EEF1AKMT1 (PubMed:26545399). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (PubMed:25144183). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (PubMed:28520920). Trimethylated at Gly-2 by METTL13 (PubMed:30143613). Mono- and dimethylated at Lys-55 by METTL13; dimethylated form is predominant (PubMed:30143613, PubMed:30612740).. Ubiquitinated at Lys-385 by RNF14 in response to ribosome collisions (ribosome stalling), leading to its degradation by the proteasome and rescue of stalled ribosomes.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed : 17177976).. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
See full target information EEF1A1
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB140632

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

  • 5
  • 2
Proteins & Peptides

AB130062

Recombinant Human OLA1 protein

SDS-PAGE - Recombinant Human OLA1 protein (AB130062)

  • 0
  • 0
Proteins & Peptides

AB322095

Recombinant Mouse Haptoglobin Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse Haptoglobin Protein Standard (His tag) (AB322095)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com