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AB181937

Recombinant Human EF-Ts protein (denatured) (Tag Free)

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Recombinant Human EF-Ts protein (denatured) (Tag Free) is a Human Full Length protein, in the 46 to 346 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

EF-Ts, EF-TsMt, TSFM

1 Images
SDS-PAGE - Recombinant Human EF-Ts protein (denatured) (Tag Free) (AB181937)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EF-Ts protein (denatured) (Tag Free) (AB181937)

15% SDS-PAGE analysis of 3 μg ab181937.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P43897

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE","proteinLength":"Full Length","predictedMolecularWeight":"32.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":346,"aminoAcidStart":46,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P43897","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EF-Ts also known as Elongation Factor Ts is a protein with a molecular mass of approximately 30 kDa. It plays a significant role in protein synthesis specifically during the elongation phase of translation. EF-Ts is a guanine nucleotide exchange factor and primarily expressed in prokaryotic cells aiding EF-Tu in the exchange of GDP for GTP. By this function EF-Ts maintains a continuous supply of active EF-Tu essential for protein translation efficiency.
Biological function summary

EF-Ts facilitates the regeneration of its partner EF-Tu supporting its function in the ribosome. This protein forms part of the elongation factor complex an essential component of the translation machinery in bacterial cells. EF-Ts ensures EF-Tu remains in its active form enabling the delivery of aminoacyl-tRNA to the ribosomal A-site. Without EF-Ts EF-Tu would remain GDP-bound hampering protein synthesis.

Pathways

EF-Ts contributes to the translational control pathway by interacting directly with EF-Tu and ribosomes. It's important for maintaining the fidelity and speed of protein synthesis. EF-Ts operates alongside these components in the protein synthesis pathway which is central to cellular function and growth. EF-Ts does not work in isolation but coordinates closely with these other translational factors to maintain efficient protein production.

Dysfunctions in protein synthesis involving EF-Ts have implications for bacterial infections and antibiotic resistance. EF-Ts can be a target for antibiotics that aim to disrupt bacterial translation. Additionally studies have shown that mutations or alterations in related proteins like EF-Tu may lead to antibiotic resistance making EF-Ts a significant protein in understanding and combating bacterial diseases. Understanding EF-Ts' precise role offers insights into novel therapeutic approaches against these infections.
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Specifications

Form

Liquid

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General info

Function

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.

Sequence similarities

Belongs to the EF-Ts family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
See full target information TSFM
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