Recombinant Human EF-Ts protein (denatured) is a Human Full Length protein, in the 46 to 346 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S K E L L M K L R R K T G Y S F V N C K K A L E T C G G D L K Q A E I W L H K E A Q K E G W S K A A K L Q G R K T K E G L I G L L Q E G N T T V L V E V N C E T D F V S R N L K F Q L L V Q Q V A L G T M M H C Q T L K D Q P S A Y S K V Q W L T P V N L A L W E A E A G G S L E G F L N S S E L S G L P A G P D R E G S L K D Q L A L A I G K L G E N M I L K R A A W V K V P S G F Y V G S Y V H G A M Q S P S L H K L V L G K Y G A L V I C E T S E Q K T N L E D V G R R L G Q H V V G M A P L S V G S L D D E P G G E A E T K M L S Q P Y L L D P S I T L G Q Y V Q P Q G V S V V D F V R F E C G E G E E A A E T E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
Alternative names
EF-Ts, EF-TsMt, TSFM
Recommended products
Recombinant Human EF-Ts protein (denatured) is a Human Full Length protein, in the 46 to 346 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P43897-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M S K E L L M K L R R K T G Y S F V N C K K A L E T C G G D L K Q A E I W L H K E A Q K E G W S K A A K L Q G R K T K E G L I G L L Q E G N T T V L V E V N C E T D F V S R N L K F Q L L V Q Q V A L G T M M H C Q T L K D Q P S A Y S K V Q W L T P V N L A L W E A E A G G S L E G F L N S S E L S G L P A G P D R E G S L K D Q L A L A I G K L G E N M I L K R A A W V K V P S G F Y V G S Y V H G A M Q S P S L H K L V L G K Y G A L V I C E T S E Q K T N L E D V G R R L G Q H V V G M A P L S V G S L D D E P G G E A E T K M L S Q P Y L L D P S I T L G Q Y V Q P Q G V S V V D F V R F E C G E G E E A A E T E
- Accession
- P43897
- Protein length
- Full Length
- Predicted molecular weight
- 32.9 kDa
- Amino acids
- 46 to 346
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
- Sequence similarities
Belongs to the EF-Ts family.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EF-Ts also known as Elongation Factor Ts is a protein with a molecular mass of approximately 30 kDa. It plays a significant role in protein synthesis specifically during the elongation phase of translation. EF-Ts is a guanine nucleotide exchange factor and primarily expressed in prokaryotic cells aiding EF-Tu in the exchange of GDP for GTP. By this function EF-Ts maintains a continuous supply of active EF-Tu essential for protein translation efficiency.
- Biological function summary
EF-Ts facilitates the regeneration of its partner EF-Tu supporting its function in the ribosome. This protein forms part of the elongation factor complex an essential component of the translation machinery in bacterial cells. EF-Ts ensures EF-Tu remains in its active form enabling the delivery of aminoacyl-tRNA to the ribosomal A-site. Without EF-Ts EF-Tu would remain GDP-bound hampering protein synthesis.
- Pathways
EF-Ts contributes to the translational control pathway by interacting directly with EF-Tu and ribosomes. It's important for maintaining the fidelity and speed of protein synthesis. EF-Ts operates alongside these components in the protein synthesis pathway which is central to cellular function and growth. EF-Ts does not work in isolation but coordinates closely with these other translational factors to maintain efficient protein production.
- Associated diseases and disorders
Dysfunctions in protein synthesis involving EF-Ts have implications for bacterial infections and antibiotic resistance. EF-Ts can be a target for antibiotics that aim to disrupt bacterial translation. Additionally studies have shown that mutations or alterations in related proteins like EF-Tu may lead to antibiotic resistance making EF-Ts a significant protein in understanding and combating bacterial diseases. Understanding EF-Ts' precise role offers insights into novel therapeutic approaches against these infections.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human EF-Ts protein (denatured) (ab181937)
15% SDS-PAGE analysis of 3 μg ab181937.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com