JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB114651

Recombinant Human EFEMP1/Fibulin-3 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human EFEMP1/Fibulin-3 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 493 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3

1 Images
SDS-PAGE - Recombinant Human EFEMP1/Fibulin-3 protein (AB114651)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EFEMP1/Fibulin-3 protein (AB114651)

12.5% SDS-PAGE showing ab114651 at approximately 79.97kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q12805

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
style
left-column

Product details

This product was previously labelled as EFEMP1.
style
left-column

Sequence info

[{"sequence":"MLKALFLTMLTLALVKSQDTEETITYTQCTDGYEWDPVGQQCKDIDECDIVPDACKGGMKCVNHYGGYLCLPKTAQIIVNNEQPQQETQPAEGTSGATTGVVAASSMATSGVLPGGGFVASAAAVAGPEMQTGRNNFVIRRNPADPQRIPSNPSHRIQCAAGYEQSEHNVCQDIDECTAGTHNCRADQVCINLRGSFACQCPPGYQKRGEQCVDIDECTIPPYCHQRCVNTPGSFYCQCSPGFQLAANNYTCVDINECDASNQCAQQCYNILGSFICQCNQGYELSSDRLNCEDIDECRTSSYLCQYQCVNEPGKFSCMCPQGYQVVRSRTCQDINECETTNECREDEMCWNYHGGFRCYPRNPCQDPYILTPENRCVCPVSNAMCRELPQSIVYKYMSIRSDRSVPSDIFQIQATTIYANTINTFRIKSGSENGEFYLRQTSPVSAMLVLVKSLSGPREHIVDLEMLTASSIGTFRTSSVLRLTIIVGPFSF","proteinLength":"Full Length","predictedMolecularWeight":"79.97 kDa","actualMolecularWeight":null,"aminoAcidEnd":493,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q12805","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EFEMP1 also known as Fibulin-3 is a member of the fibulin family involved in extracellular matrix (ECM) organization. The protein's mass is approximately 55 kDa. It is expressed in various tissues but researchers find it most common in the eye lung and arterial walls. As a secreted glycoprotein Fibulin-3 plays a vital role in maintaining structural integrity and elasticity in connective tissues.
Biological function summary

EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.

Pathways

EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.

EFEMP1/Fibulin-3 has notable connections with certain pathologies. Malfunctions in its expression or structure can relate to age-related macular degeneration and various forms of cancer including mesothelioma. In these diseases Fibulin-3 interacts with proteins such as matrix metalloproteinases which degrade extracellular matrix proteins thereby influencing tumor progression and ECM remodeling in affected tissues.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

Sequence similarities

Belongs to the fibulin family.

style
left-column

Product protocols

style
left-column

Target data

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
See full target information EFEMP1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB256457

Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] (AB256457)

  • 3
  • 1
Assay Kits

AB269552

Human EFEMP1 ELISA Kit (Fibulin-3)

Recombinant|SimpleStep

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

  • 0
  • 0
Proteins & Peptides

AB204627

Recombinant Human Wnt5a protein

SDS-PAGE - Recombinant Human Wnt5a protein (AB204627)

  • 0
  • 0
Proteins & Peptides

AB319985

Recombinant Mouse Prealbumin Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse Prealbumin Protein Standard (His tag) (AB319985)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com