Recombinant Human EFEMP1/Fibulin-3 protein (His tag) is a Human Full Length protein, in the 18 to 493 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
Q D T E E T I T Y T Q C T D G Y E W D P V R Q Q C K D I D E C D I V P D A C K G G M K C V N H Y G G Y L C L P K T A Q I I V N N E Q P Q Q E T Q P A E G T S G A T T G V V A A S S M A T S G V L P G G G F V A S A A A V A G P E M Q T G R N N F V I R R N P A D P Q R I P S N P S H R I Q C A A G Y E Q S E H N V C Q D I D E C T A G T H N C R A D Q V C I N L R G S F A C Q C P P G Y Q K R G E Q C V D I D E C T I P P Y C H Q R C V N T P G S F Y C Q C S P G F Q L A A N N Y T C V D I N E C D A S N Q C A Q Q C Y N I L G S F I C Q C N Q G Y E L S S D R L N C E D I D E C R T S S Y L C Q Y Q C V N E P G K F S C M C P Q G Y Q V V R S R T C Q D I N E C E T T N E C R E D E M C W N Y H G G F R C Y P R N P C Q D P Y I L T P E N R C V C P V S N A M C R E L P Q S I V Y K Y M S I R S D R S V P S D I F Q I Q A T T I Y A N T I N T F R I K S G N E N G E F Y L R Q T S P V S A M L V L V K S L S G P R E H I V D L E M L T V S S I G T F R T S S V L R L T I I V G P F S F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Alternative names
FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3
Recommended products
Recombinant Human EFEMP1/Fibulin-3 protein (His tag) is a Human Full Length protein, in the 18 to 493 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- Q12805-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Q D T E E T I T Y T Q C T D G Y E W D P V R Q Q C K D I D E C D I V P D A C K G G M K C V N H Y G G Y L C L P K T A Q I I V N N E Q P Q Q E T Q P A E G T S G A T T G V V A A S S M A T S G V L P G G G F V A S A A A V A G P E M Q T G R N N F V I R R N P A D P Q R I P S N P S H R I Q C A A G Y E Q S E H N V C Q D I D E C T A G T H N C R A D Q V C I N L R G S F A C Q C P P G Y Q K R G E Q C V D I D E C T I P P Y C H Q R C V N T P G S F Y C Q C S P G F Q L A A N N Y T C V D I N E C D A S N Q C A Q Q C Y N I L G S F I C Q C N Q G Y E L S S D R L N C E D I D E C R T S S Y L C Q Y Q C V N E P G K F S C M C P Q G Y Q V V R S R T C Q D I N E C E T T N E C R E D E M C W N Y H G G F R C Y P R N P C Q D P Y I L T P E N R C V C P V S N A M C R E L P Q S I V Y K Y M S I R S D R S V P S D I F Q I Q A T T I Y A N T I N T F R I K S G N E N G E F Y L R Q T S P V S A M L V L V K S L S G P R E H I V D L E M L T V S S I G T F R T S S V L R L T I I V G P F S F
- Accession
- Q12805
- Protein length
- Full Length
- Predicted molecular weight
- 54.67 kDa
- Amino acids
- 18 to 493
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
- Sequence similarities
Belongs to the fibulin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Notes
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EFEMP1 also known as Fibulin-3 is a member of the fibulin family involved in extracellular matrix (ECM) organization. The protein's mass is approximately 55 kDa. It is expressed in various tissues but researchers find it most common in the eye lung and arterial walls. As a secreted glycoprotein Fibulin-3 plays a vital role in maintaining structural integrity and elasticity in connective tissues.
- Biological function summary
EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.
- Pathways
EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.
- Associated diseases and disorders
EFEMP1/Fibulin-3 has notable connections with certain pathologies. Malfunctions in its expression or structure can relate to age-related macular degeneration and various forms of cancer including mesothelioma. In these diseases Fibulin-3 interacts with proteins such as matrix metalloproteinases which degrade extracellular matrix proteins thereby influencing tumor progression and ECM remodeling in affected tissues.
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3 product images
SDS-PAGE - Recombinant Human EFEMP1/Fibulin-3 protein (His tag) (ab318255)
SDS-PAGE analysis of ab318255 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Human EFEMP1/Fibulin-3 protein (His tag) (ab318255)
Mass spectrometry (MS/MS) analysis of ab318255
HPLC - Recombinant Human EFEMP1/Fibulin-3 protein (His tag) (ab318255)
HPLC analysis of ab318255
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