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AB242209

Recombinant human EG-VEGF protein (Active)

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Recombinant human EG-VEGF protein (Active) is a Human Full Length protein, in the 20 to 105 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

UNQ600/PRO1186, PROK1, Prokineticin-1, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, EG-VEGF

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SDS-PAGE - Recombinant human EG-VEGF protein (Active) (AB242209)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human EG-VEGF protein (Active) (AB242209)

SDS-PAGE analysis of ab242209.

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using bovine EJG cells is less than 2.0 μg/ml, corresponding to a specific activity of >500 IU/mg.

Accession

P58294

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water or 0.1% BSA aqueous buffer

Storage buffer

Constituents: PBS, 0.02% Sorbitan monolaurate, ethoxylated

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AVITGACERDVQCGAGTCCAISLWLRGLRMCTPLGREGEECHPGSHKVPFFRKRKHHTCPCLPNLLCSRFPDGRYRCSMDLKNINF","proteinLength":"Full Length","predictedMolecularWeight":"9.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":105,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P58294","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EG-VEGF also known as endocrine gland-derived vascular endothelial growth factor or prokineticin 1 is a potent angiogenic mitogen. It typically has a molecular mass of around 9 kDa. EG-VEGF is expressed mainly in steroidogenic tissues such as the ovaries testes adrenal glands and placenta. It targets endothelial cells selectively within these glands inducing various responses like proliferation migration and fenestration. This selectivity makes it distinct from vascular endothelial growth factor A (VEGF-A) which has a broader expression pattern.
Biological function summary

EG-VEGF plays a critical role in the formation and remodeling of blood vessels specific to endocrine glands. It is not part of a larger protein complex but operates through interaction with its receptor prokineticin receptor 1 (PROKR1). This unique interaction means that it has a specific ligand-receptor activity contributing to the regulation of vascular development especially in tissues undergoing hormonal changes. This makes EG-VEGF significant in contexts where localized and specific angiogenesis is essential.

Pathways

EG-VEGF is important in the regulation of angiogenesis and endothelial function. It fits into pathways that influence cell signaling associated with vascular development. One such pathway is the hypoxia-inducible factor (HIF) pathway which enhances the expression of EG-VEGF under low oxygen conditions. Another related protein is vascular endothelial growth factor (VEGF) whereby VEGF and EG-VEGF demonstrate distinctive yet occasionally overlapping functions in angiogenesis optimizing vascular growth within specific environments.

EG-VEGF shows associations with conditions like preeclampsia and polycystic ovary syndrome (PCOS). Preeclampsia involves improper vascularization in the placenta where EG-VEGF's role in angiogenesis becomes critical as it may help or disrupt proper vascular development. Polycystic ovary syndrome a hormonal disorder impacting ovaries also links with EG-VEGF through its effect on ovarian blood vessels. These disorders highlight potential therapeutic targets considering EG-VEGF's integration with other proteins like PROKR1 which could modulate its angiogenic functions.
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Specifications

Form

Lyophilized

Additional notes

> 98 % by HPLC.

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General info

Function

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.

Sequence similarities

Belongs to the AVIT (prokineticin) family.

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Product protocols

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Target data

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
See full target information PROK1
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