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Recombinant human EGF protein (Active) is a Human Full Length protein, in the 971 to 1023 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.


Images

Functional Studies - Recombinant human EGF protein (Active) (AB259398), expandable thumbnail
  • SDS-PAGE - Recombinant human EGF protein (Active) (AB259398), expandable thumbnail
  • HPLC - Recombinant human EGF protein (Active) (AB259398), expandable thumbnail
  • Mass Spectrometry - Recombinant human EGF protein (Active) (AB259398), expandable thumbnail

Publications

Key facts

Purity
>=95% SDS-PAGE
Endotoxin level
< 0.005 EU/µg
Expression system
HEK 293 cells
Tags
Tag free
Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
Biologically active
Yes

Amino acid sequence

N S D S E C P L S H D G Y C L H D G V C M Y I E A L D K Y A C N C V V G Y I G E R C Q Y R D L K W W E L R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Alternative names

Recommended products

Recombinant human EGF protein (Active) is a Human Full Length protein, in the 971 to 1023 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Key facts

Purity
>=95% SDS-PAGE
Endotoxin level
< 0.005 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of BALB / 3T3 cells is 0.99 ng/mL corresponding to a Specific Activity of 1.01 x 106 IU/mg.
Accession
P01133-1
Animal free
Yes
Species
Human
Reconstitution
Reconstitute in PBS
Concentration
Loading...
Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

N S D S E C P L S H D G Y C L H D G V C M Y I E A L D K Y A C N C V V G Y I G E R C Q Y R D L K W W E L R
Accession
P01133
Protein length
Full Length
Predicted molecular weight
6.12 kDa
Amino acids
971 to 1023
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

>= 95 % HPLC.

General info

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Post-translational modifications

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.

Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Associated diseases and disorders

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.

Product promise

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4 product images

  • Functional Studies - Recombinant human EGF protein (Active) (ab259398), expandable thumbnail

    Functional Studies - Recombinant human EGF protein (Active) (ab259398)

    Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of BALB / 3T3 cells is 0.99 ng/mL corresponding to a Specific Activity of 1.01 x 106 IU/mg.

  • SDS-PAGE - Recombinant human EGF protein (Active) (ab259398), expandable thumbnail

    SDS-PAGE - Recombinant human EGF protein (Active) (ab259398)

    SDS-PAGE analysis of ab259398.

  • HPLC - Recombinant human EGF protein (Active) (ab259398), expandable thumbnail

    HPLC - Recombinant human EGF protein (Active) (ab259398)

    Purity: 97%

    The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.

  • Mass Spectrometry - Recombinant human EGF protein (Active) (ab259398), expandable thumbnail

    Mass Spectrometry - Recombinant human EGF protein (Active) (ab259398)

    (M-Arg) +/- 0.1 Da (loss of C-terminal Arg; calc. mass 6123 Da)

    The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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