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Recombinant human EGF protein (Animal Free) is a Human Full Length protein, in the 971 to 1023 aa range, expressed in Escherichia coli, with >=98% purity, < 0.01 EU/µg endotoxin level and suitable for SDS-PAGE, WB, HPLC, CellAct.
Alternative names=Pro-epidermal growth factor, EGF, EGF
- Purity
>=98% SDS-PAGE
- Endotoxin level
< 0.01 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, WB, HPLC, CellAct
- Biologically active
Yes
- Sequence
N S D S E C P L S H D G Y C L H D G V C M Y I E A L D K Y A C N C V V G Y I G E R C Q Y R D L K W W E L R
Consider this alternative
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application CellAct | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human EGF protein (Animal Free) is a Human Full Length protein, in the 971 to 1023 aa range, expressed in Escherichia coli, with >=98% purity, < 0.01 EU/µg endotoxin level and suitable for SDS-PAGE, WB, HPLC, CellAct.
Alternative names=Pro-epidermal growth factor, EGF, EGF
Key facts
- Purity
>=98% SDS-PAGE
- Endotoxin level
< 0.01 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, WB, HPLC, CellAct
- Biologically active
Yes
- Biological activity
The ED50,as determined by a cell proliferation assay using balb/c 3T3 cells, is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Predicted molecular weight
6.2 kDa
- Amino acids
971 to 1023
- Sequence
N S D S E C P L S H D G Y C L H D G V C M Y I E A L D K Y A C N C V V G Y I G E R C Q Y R D L K W W E L R
- Accession
- P01133-1
- Protein length
Full Length
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.
- Concentration
Specifications
- Form
Lyophilized
- Additional notes
Sterile filtered.Greater than 98% pure by HPLC analyses.
General info
- Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
- Post-translational modifications
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Manufactured using all non-animal reagents.
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