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Recombinant human EGFR protein (Active) is a Human Fragment protein, in the 25 to 645 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

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Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, ELISA, FuncS

Biologically active

Yes

Amino acid sequence

L E E K K V C Q G T S N K L T Q L G T F E D H F L S L Q R M F N N C E V V L G N L E I T Y V Q R N Y D L S F L K T I Q E V A G Y V L I A L N T V E R I P L E N L Q I I R G N M Y Y E N S Y A L A V L S N Y D A N K T G L K E L P M R N L Q E I L H G A V R F S N N P A L C N V E S I Q W R D I V S S D F L S N M S M D F Q N H L G S C Q K C D P S C P N G S C W G A G E E N C Q K L T K I I C A Q Q C S G R C R G K S P S D C C H N Q C A A G C T G P R E S D C L V C R K F R D E A T C K D T C P P L M L Y N P T T Y Q M D V N P E G K Y S F G A T C V K K C P R N Y V V T D H G S C V R A C G A D S Y E M E E D G V R K C K K C E G P C R K V C N G I G I G E F K D S L S I N A T N I K H F K N C T S I S G D L H I L P V A F R G D S F T H T P P L D P Q E L D I L K T V K E I T G F L L I Q A W P E N R T D L H A F E N L E I I R G R T K Q H G Q F S L A V V S L N I T S L G L R S L K E I S D G D V I I S G N K N L C Y A N T I N W K K L F G T S G Q K T K I I S N R G E N S C K A T G Q V C H A L C S P E G C W G P E P R D C V S C R N V S R G R E C V D K C N L L E G E P R E F V E N S E C I Q C H P E C L P Q A M N I T C T G R G P D N C I Q C A H Y I D G P H C V K T C P A G V M G E N N T L V W K Y A D A G H V C H L C H P N C T Y G C T G P G L E G C P T N G P K I P S

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity).Isoform 2 may act as an antagonist of EGF action.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Alternative names

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Recombinant human EGFR protein (Active) is a Human Fragment protein, in the 25 to 645 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, ELISA, FuncS

Biological activity

Measured by its binding ability in a functional ELISA.

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind ab155639 with a linear range of 0.006-0.025 μg/mL.

Accession
P00533-1
Animal free

No

Species

Human

Reconstitution

For lot specific reconstitution information please contact our Scientific Support Team.

Concentration
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Storage buffer

pH: 7.4
Constituents: PBS, 5% Trehalose

Sequence info

Amino acid sequence

L E E K K V C Q G T S N K L T Q L G T F E D H F L S L Q R M F N N C E V V L G N L E I T Y V Q R N Y D L S F L K T I Q E V A G Y V L I A L N T V E R I P L E N L Q I I R G N M Y Y E N S Y A L A V L S N Y D A N K T G L K E L P M R N L Q E I L H G A V R F S N N P A L C N V E S I Q W R D I V S S D F L S N M S M D F Q N H L G S C Q K C D P S C P N G S C W G A G E E N C Q K L T K I I C A Q Q C S G R C R G K S P S D C C H N Q C A A G C T G P R E S D C L V C R K F R D E A T C K D T C P P L M L Y N P T T Y Q M D V N P E G K Y S F G A T C V K K C P R N Y V V T D H G S C V R A C G A D S Y E M E E D G V R K C K K C E G P C R K V C N G I G I G E F K D S L S I N A T N I K H F K N C T S I S G D L H I L P V A F R G D S F T H T P P L D P Q E L D I L K T V K E I T G F L L I Q A W P E N R T D L H A F E N L E I I R G R T K Q H G Q F S L A V V S L N I T S L G L R S L K E I S D G D V I I S G N K N L C Y A N T I N W K K L F G T S G Q K T K I I S N R G E N S C K A T G Q V C H A L C S P E G C W G P E P R D C V S C R N V S R G R E C V D K C N L L E G E P R E F V E N S E C I Q C H P E C L P Q A M N I T C T G R G P D N C I Q C A H Y I D G P H C V K T C P A G V M G E N N T L V W K Y A D A G H V C H L C H P N C T Y G C T G P G L E G C P T N G P K I P S

Accession

P00533

Protein length

Fragment

Predicted molecular weight

70.5 kDa

Amino acids

25 to 645

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

General info

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Post-translational modifications

Phosphorylated on Tyr residues in response to EGF (PubMed:20462955, PubMed:27153536). Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.

Subcellular localisation

Nucleus membrane, Endosome, Endosome membrane, Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Activity summary

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

Associated diseases and disorders

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - Recombinant human EGFR protein (Active) (ab155639), expandable thumbnail

    Functional Studies - Recombinant human EGFR protein (Active) (ab155639)

    Erbitux (Cetuximab), captured on CM5 chip via anti-human IgG Fc antibodies surface, binds ab155639 with an affinity constant of 1.3 nM, as determined in an SPR assay (Biacore T200) (Routinely tested).

  • SDS-PAGE - Recombinant human EGFR protein (Active) (ab155639), expandable thumbnail

    SDS-PAGE - Recombinant human EGFR protein (Active) (ab155639)

    Reduced ab155639 on SDS-PAGE, stained overnight with Coomassie Blue.

    The purity of the protein is greater than 95%.

    The protein migrates as 95-120 kDa under reducing condition due to glycosylation.

  • Functional Studies - Recombinant human EGFR protein (Active) (ab155639), expandable thumbnail

    Functional Studies - Recombinant human EGFR protein (Active) (ab155639)

    Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind ab155639 with a linear range of 0.006-0.025 μg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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