Recombinant human EGFR protein (Active)

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, ELISA, FuncS

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA.

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind ab155639 with a linear range of 0.006-0.025 μg/mL.

Predicted molecular weight

70.5 kDa

Amino acids

25 to 645

Sequence

L E E K K V C Q G T S N K L T Q L G T F E D H F L S L Q R M F N N C E V V L G N L E I T Y V Q R N Y D L S F L K T I Q E V A G Y V L I A L N T V E R I P L E N L Q I I R G N M Y Y E N S Y A L A V L S N Y D A N K T G L K E L P M R N L Q E I L H G A V R F S N N P A L C N V E S I Q W R D I V S S D F L S N M S M D F Q N H L G S C Q K C D P S C P N G S C W G A G E E N C Q K L T K I I C A Q Q C S G R C R G K S P S D C C H N Q C A A G C T G P R E S D C L V C R K F R D E A T C K D T C P P L M L Y N P T T Y Q M D V N P E G K Y S F G A T C V K K C P R N Y V V T D H G S C V R A C G A D S Y E M E E D G V R K C K K C E G P C R K V C N G I G I G E F K D S L S I N A T N I K H F K N C T S I S G D L H I L P V A F R G D S F T H T P P L D P Q E L D I L K T V K E I T G F L L I Q A W P E N R T D L H A F E N L E I I R G R T K Q H G Q F S L A V V S L N I T S L G L R S L K E I S D G D V I I S G N K N L C Y A N T I N W K K L F G T S G Q K T K I I S N R G E N S C K A T G Q V C H A L C S P E G C W G P E P R D C V S C R N V S R G R E C V D K C N L L E G E P R E F V E N S E C I Q C H P E C L P Q A M N I T C T G R G P D N C I Q C A H Y I D G P H C V K T C P A G V M G E N N T L V W K Y A D A G H V C H L C H P N C T Y G C T G P G L E G C P T N G P K I P S

Accession

P00533-1

Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

For lot specific reconstitution information please contact our Scientific Support Team.

Concentration

Storage buffer

pH: 7.4
Constituents: PBS, 5% Trehalose

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