Recombinant Human EHD2 protein is a Human Full Length protein, in the 1 to 543 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M F S W L K R G G A R G Q Q P E A I R T V T S A L K E L Y R T K L L P L E E H Y R F G A F H S P A L E D A D F D G K P M V L V A G Q Y S T G K T S F I Q Y L L E Q E V P G S R V G P E P T T D C F V A V M H G D T E G T V P G N A L V V D P D K P F R K L N P F G N T F L N R F M C A Q L P N Q V L E S I S I I D T P G I L S G A K Q R V S R G Y D F P A V L R W F A E R V D L I I L L F D A H K L E I S D E F S E A I G A L R G H E D K I R V V L N K A D M V E T Q Q L M R V Y G A L M W A L G K V V G T P E V L R V Y I G S F W S Q P L L V P D N R R L F E L E E Q D L F R D I Q G L P R H A A L R K L N D L V K R A R L V R V H A Y I I S Y L K K E M P S V F G K E N K K K Q L I L K L P V I F A K I Q L E H H I S P G D F P D C Q K M Q E L L M A H D F T K F H S L K P K L L E A L D E M L T H D I A K L M P L L R Q E E L E S T E V G V Q G G A F E G T H M G P F V E R G P D E A M E D G E E G S D D E A E W V V T K D K S K Y D E I F Y N L A P A D G K L S G S K A K T W M V G T K L P N S V L G R I W K L S D V D R D G M L D D E E F A L A S H L I E A K L E G H G L P A N L P R R L V P P S K R R H K G S A E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed:25588833).
Alternative names
PAST2, EHD2, EH domain-containing protein 2, PAST homolog 2
Recommended products
Recombinant Human EHD2 protein is a Human Full Length protein, in the 1 to 543 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9NZN4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M F S W L K R G G A R G Q Q P E A I R T V T S A L K E L Y R T K L L P L E E H Y R F G A F H S P A L E D A D F D G K P M V L V A G Q Y S T G K T S F I Q Y L L E Q E V P G S R V G P E P T T D C F V A V M H G D T E G T V P G N A L V V D P D K P F R K L N P F G N T F L N R F M C A Q L P N Q V L E S I S I I D T P G I L S G A K Q R V S R G Y D F P A V L R W F A E R V D L I I L L F D A H K L E I S D E F S E A I G A L R G H E D K I R V V L N K A D M V E T Q Q L M R V Y G A L M W A L G K V V G T P E V L R V Y I G S F W S Q P L L V P D N R R L F E L E E Q D L F R D I Q G L P R H A A L R K L N D L V K R A R L V R V H A Y I I S Y L K K E M P S V F G K E N K K K Q L I L K L P V I F A K I Q L E H H I S P G D F P D C Q K M Q E L L M A H D F T K F H S L K P K L L E A L D E M L T H D I A K L M P L L R Q E E L E S T E V G V Q G G A F E G T H M G P F V E R G P D E A M E D G E E G S D D E A E W V V T K D K S K Y D E I F Y N L A P A D G K L S G S K A K T W M V G T K L P N S V L G R I W K L S D V D R D G M L D D E E F A L A S H L I E A K L E G H G L P A N L P R R L V P P S K R R H K G S A E
- Accession
- Q9NZN4
- Protein length
- Full Length
- Predicted molecular weight
- 87.6 kDa
- Amino acids
- 1 to 543
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed:25588833).
- Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EHD2 also known as EH domain-containing protein 2 is a mechanochemical protein with a molecular mass of approximately 61 kDa. It belongs to the dynamin superfamily of GTPases. EHD2 is frequently expressed in muscle cells adipocytes and cardiac tissues. It associates with cellular membranes particularly near caveolae where it plays a pivotal role in regulating cellular membrane dynamics and endocytosis through binding to phosphoinositides.
- Biological function summary
The protein connection with membranes affects the regulation of vesicle trafficking and endosomal recycling. EHD2 functions as part of a complex with other proteins like pacsin and syndapin which modulates curvature of membranes a process essential for diverse cellular activities. By facilitating endocytosis EHD2 has a role in signaling pathways that influence cell migration and membrane repair.
- Pathways
EHD2 interacts significantly with lipid raft-associated pathways which are critical in cellular communication and signal transduction. This protein interacts with the caveolin protein family particularly Caveolin-1 during caveolae-mediated endocytosis. It contributes to the spatial organization of signaling domains impacting the cellular response to external stimuli and stress.
- Associated diseases and disorders
The malfunction or altered expression of EHD2 associates with disorders such as obesity and muscle-related diseases. EHD2 dysregulation can affect Caveolin-1 contributing to abnormal vesicular transport and lipid metabolism. Such dysfunction holds potential links to the development or progression of conditions like cardiac hypertrophy highlighting the protein's importance in cellular health and disease.
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1 product image
SDS-PAGE - Recombinant Human EHD2 protein (ab132288)
12.5% SDS-PAGE stained with Coomassie Blue showing ab132288.
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