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AB268519

Recombinant human EHMT1/GLP protein (Active)

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Recombinant human EHMT1/GLP protein (Active) is a Human Fragment protein, in the 895 to 1298 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

EUHMTASE1, GLP, KIAA1876, KMT1D, EHMT1, Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1, G9a-like protein 1, Histone H3-K9 methyltransferase 5, Lysine N-methyltransferase 1D, Eu-HMTase1, GLP1, H3-K9-HMTase 5

2 Images
Functional Studies - Recombinant human EHMT1/GLP protein (Active) (AB268519)
  • FuncS

Supplier Data

Functional Studies - Recombinant human EHMT1/GLP protein (Active) (AB268519)

The specific activity of ab268519 was 380 nmol/min/mg in a methyltransferase assay using histone H3 peptide (1-21) as substrate.

SDS-PAGE - Recombinant human EHMT1/GLP protein (Active) (AB268519)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human EHMT1/GLP protein (Active) (AB268519)

SDS-PAGE analysis of ab268519.

MW ~74 kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268519 was 380 nmol/min/mg in a methyltransferase assay using histone H3 peptide (1-21) as substrate.

Accession

Q9H9B1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCPSNYKYVSQNCVTSPMNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTRDMGWGVRSLQDIPPGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMAHQDLRFPRIAFFSTRLIEAGEQLGFDYGERFWDIKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQEDGLPDTSSAAAADPL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1298,"aminoAcidStart":895,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9H9B1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHMT1 also known as GLP (G9a-like protein) is a histone methyltransferase involved in the methylation of histone H3 on lysine 9 a modification leading to transcriptional repression. The EHMT1 protein has a mass of approximately 150 kDa and is expressed in numerous tissues including the brain and heart. It functions as part of a larger complex and acts in the modification of chromatin structure and gene expression regulation.
Biological function summary

EHMT1/GLP plays a critical role in cellular processes such as development and differentiation. It forms a complex with G9a another histone methyltransferase to facilitate these functions. This complex cooperates in controlling gene silencing and the preservation of cellular identity. Such complex mechanisms lead to alterations in gene expression patterns that are essential for normal cellular operation.

Pathways

The methylation activity of EHMT1/GLP participates in the epigenetic regulation pathway and chromatin remodeling pathways. Within these pathways it interacts closely with proteins like HP1 which binds to methylated histones to promote gene silencing. The connections with pathways that influence gene expression affirm its role in maintaining cellular processes like proliferation and embryonic development.

Researchers connect EHMT1/GLP to Kleefstra syndrome—a condition characterized by intellectual disability and developmental delays. It is also associated with some cancer types due to its epigenetic regulation influences. In these contexts abnormal EHMT1/GLP activity often involving other proteins like G9a can contribute to the disruptions in gene regulation that lead to disease manifestations.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins : mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B (By similarity).

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins : mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B (By similarity).
See full target information EHMT1
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