Recombinant human EHMT2/G9A protein is a Human Fragment protein, in the 807 to 1210 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
R G A D V T L T D N E E N I C L H W A S F T G S A A I A E V L L N A R C D L H A V N Y H G D T P L H I A A R E S Y H D C V L L F L S R G A N P E L R N K E G D T A W D L T P E R S D V W F A L Q L N R K L R L G V G N R A I R T E K I I C R D V A R G Y E N V P I P C V N G V D G E P C P E D Y K Y I S E N C E T S T M N I D R N I T H L Q H C T C V D D C S S S N C L C G Q L S I R C W Y D K D G R L L Q E F N K I E P P L I F E C N Q A C S C W R N C K N R V V Q S G I K V R L Q L Y R T A K M G W G V R A L Q T I P Q G T F I C E Y V G E L I S D A E A D V R E D D S Y L F D L D N K D G E V Y C I D A R Y Y G N I S R F I N H L C D P N I I P V R V F M L H Q D L R F P R I A F F S S R D I R T G E E L G F D Y G D R F W D I K S K Y F T C Q C G S E K C K H S A E A I A L E Q S R L A R L D P H P E L L P E L G S L P P V N T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Alternative names
BAT8, C6orf30, G9A, KMT1C, NG36, EHMT2, Histone-lysine N-methyltransferase EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Lysine N-methyltransferase 1C, Protein G9a, H3-K9-HMTase 3
Recommended products
Recombinant human EHMT2/G9A protein is a Human Fragment protein, in the 807 to 1210 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab167966 was determined to be 15 nmol/min/mg as per activity assay protocol.
- Accession
- Q96KQ7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- R G A D V T L T D N E E N I C L H W A S F T G S A A I A E V L L N A R C D L H A V N Y H G D T P L H I A A R E S Y H D C V L L F L S R G A N P E L R N K E G D T A W D L T P E R S D V W F A L Q L N R K L R L G V G N R A I R T E K I I C R D V A R G Y E N V P I P C V N G V D G E P C P E D Y K Y I S E N C E T S T M N I D R N I T H L Q H C T C V D D C S S S N C L C G Q L S I R C W Y D K D G R L L Q E F N K I E P P L I F E C N Q A C S C W R N C K N R V V Q S G I K V R L Q L Y R T A K M G W G V R A L Q T I P Q G T F I C E Y V G E L I S D A E A D V R E D D S Y L F D L D N K D G E V Y C I D A R Y Y G N I S R F I N H L C D P N I I P V R V F M L H Q D L R F P R I A F F S S R D I R T G E E L G F D Y G D R F W D I K S K Y F T C Q C G S E K C K H S A E A I A L E Q S R L A R L D P H P E L L P E L G S L P P V N T
- Accession
- Q96KQ7
- Protein length
- Fragment
- Predicted molecular weight
- 75 kDa
- Amino acids
- 807 to 1210
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Assessed by densitometry. Affinity purified.
General info
- Function
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
- Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
- Post-translational modifications
Methylated at Lys-185; automethylated.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EHMT2 also known as G9a is a methyltransferase enzyme with a molecular mass of approximately 165 kDa. It modifies histones by adding methyl groups to lysine 9 on histone H3 which results in chromatin structure changes and influences gene expression. EHMT2/G9a expresses in various tissues including brain heart and liver showing its widespread role in different physiological processes. As a well-studied protein researchers often analyze EHMT2 activity using assays like ELISA and western blot.
- Biological function summary
EHMT2/G9a regulates gene expression by altering chromatin accessibility. It is part of a chromatin remodeling complex interacting with proteins such as ANMTA02. Through its methyltransferase activity it maintains an epigenetic silence state important for embryonic development differentiation and cellular identity. EHMT2/G9a plays a significant role in the repression of transcriptional activity of tumor suppressor genes and developmental genes.
- Pathways
G9a functions in epigenetic signaling pathways and influences processes like the cell cycle and DNA damage repair. It interacts with proteins in the Polycomb repressive complex such as EZH2 to regulate gene silencing. Additionally in the DNA damage response pathway G9a can associate with histone deacetylases to stabilize chromatin and prevent unwarranted gene expression.
- Associated diseases and disorders
Aberrant regulation of EHMT2/G9a associates with cancer and neurodevelopmental disorders. In various cancers such as leukemia G9a suppresses tumor suppressor genes aiding tumor progression. Connections between G9a and proteins like DNMT1 highlight its role in maintaining a hypermethylation state in cancerous cells. In neurodevelopmental disorders altered expression of G9a affects synaptic plasticity linking it with cognitive impairments and autism spectrum disorders.
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2 product images
SDS-PAGE - Recombinant human EHMT2/G9A protein (ab167966)
SDS Page analysis of ab167966
Functional Studies - Recombinant human EHMT2/G9A protein (ab167966)
The specific activity of ab167966 was determined to be 15 nmol/min/mg as per activity assay protocol.
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