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AB127076

Recombinant Human eIF2B4 protein (His-DHFR)

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Recombinant Human eIF2B4 protein (His-DHFR) is a Human Fragment protein, in the 319 to 523 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

EIF2BD, EIF2B4, Translation initiation factor eIF2B subunit delta, eIF2B GDP-GTP exchange factor subunit delta

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UI10

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":523,"aminoAcidStart":319,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UI10","tags":[{"tag":"His-DHFR","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2B4 also known as translation initiation factor eIF-2B subunit delta is an important component of the eIF2B complex. It plays a critical role in the regulation of protein synthesis by facilitating the exchange of GDP for GTP on eIF2. eIF2B4 has a molecular mass of approximately 67 kDa. This protein is ubiquitously expressed in various tissues indicating its importance in general cellular function.
Biological function summary

EIF2B4 functions within the eIF2B complex which is essential for the initiation of translation in eukaryotic cells. Its role in this complex involves the regulation of the eIF2 cycle which is important for controlling the rate of global protein synthesis. By mediating guanine nucleotide exchange on eIF2 eIF2B4 helps manage cellular responses to stress including the integrated stress response. This complex is vital in maintaining cellular homeostasis especially under conditions of amino acid deprivation or other stress factors.

Pathways

EIF2B4 plays a pivotal role in the integrated stress response (ISR) and is a part of the eukaryotic initiation factor 2 (eIF2) signaling pathway. These pathways adjust protein synthesis rates in response to varying environmental conditions and stress signals. eIF2B4 interacts with eIF2 and together they regulate translation by modulating the availability of eIF2-GTP. This interaction is essential for the precise control of protein synthesis during stress conditions which decides cell survival and adaptation.

EIF2B4 has a significant connection to Vanishing White Matter Disease (VWMD) and neurological disorders. Mutations in eIF2B4 can disrupt normal protein synthesis which leads to the onset of VWMD a progressive leukoencephalopathy. This pathology links eIF2B4 to disorders affecting the central nervous system. Additionally the protein relates to eIF2B5 within the context of these diseases since eIF2B5 mutations also contribute to similar pathologies suggesting their interaction is critical to neurological health.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed : 25858979, PubMed : 27023709, PubMed : 31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed : 25858979, PubMed : 31048492).

Sequence similarities

Belongs to the eIF-2B alpha/beta/delta subunits family.

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Product protocols

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Target data

Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed : 25858979, PubMed : 27023709, PubMed : 31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed : 25858979, PubMed : 31048492).
See full target information EIF2B4
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