Recombinant Human EIF3S1/EIF3J protein
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Recombinant Human EIF3S1/EIF3J protein is a Human Fragment protein, in the 70 to 258 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
EIF3S1, PRO0391, EIF3J, Eukaryotic translation initiation factor 3 subunit J, eIF3j, Eukaryotic translation initiation factor 3 subunit 1, eIF-3-alpha, eIF3 p35
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human EIF3S1/EIF3J protein (AB113578)
ab113578 at 3 μg analysed by SDS PAGE.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The eIF3J protein functions as part of the eIF3 complex to facilitate the formation of the 43S pre-initiation complex. It assists in stabilizing the interaction between the small ribosomal subunit and mRNA enhancing the accuracy and efficiency of translation initiation. The eIF3 complex is composed of 13 subunits and EIF3J contributes to the structural integrity and functionality of this complex making it essential for proper translation initiation.
Pathways
EIF3J participates in the mTOR signaling pathway and the MAPK signaling pathway. These pathways regulate cell growth proliferation and survival. In the mTOR pathway the eIF3 complex including EIF3J associates with proteins like mTORC1 to influence protein synthesis and cell growth. In the MAPK pathway EIF3J helps control translation in response to extracellular stimuli through its involvement in the eIF3 complex.
Specifications
Form
Liquid
Additional notes
ab113578 is purified using conventional chromatography techniques.
General info
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).
Sequence similarities
Belongs to the eIF-3 subunit J family.
Post-translational modifications
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
Target data
Complementary Products
Product promise
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