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AB171491

Recombinant Human EIF3S2 protein (denatured) (His tag N-Terminus)

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Recombinant Human EIF3S2 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 325 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

EIF3S2, TRIP1, EIF3I, Eukaryotic translation initiation factor 3 subunit I, eIF3i, Eukaryotic translation initiation factor 3 subunit 2, TGF-beta receptor-interacting protein 1, eIF-3-beta, eIF3 p36, TRIP-1

1 Images
SDS-PAGE - Recombinant Human EIF3S2 protein (denatured) (His tag N-Terminus) (AB171491)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EIF3S2 protein (denatured) (His tag N-Terminus) (AB171491)

15% SDS-PAGE analysis of 3μg ab171491.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q13347

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA","proteinLength":"Full Length","predictedMolecularWeight":"38.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":325,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13347","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3S2 also known as eukaryotic translation initiation factor 3 subunit 2 plays an important mechanical role in the initiation phase of protein synthesis. This protein is part of the eIF3 complex important for the formation of the translation initiation complex. EIF3S2 has a molecular weight of approximately 35 kilodaltons. The expression of EIF3S2 can be observed in various tissues including the heart liver and skeletal muscle indicating its widespread importance in cellular processes.
Biological function summary

EIF3S2 is essential for the regulation of mRNA translation. As a subunit of the eIF3 complex it helps recruit the 40S ribosomal subunit to the mRNA. This recruitment facilitates the proper scanning of mRNA ensuring accurate start codon recognition during protein synthesis. The eIF3 complex works with other initiation factors forming an important part of the translation initiation machinery. EIF3S2's role in mRNA turnover and cellular growth processes reveals its importance in maintaining normal cellular functions and protein homeostasis.

Pathways

EIF3S2 functions predominantly in protein synthesis and cell growth. It is an integral component of the mTOR signaling pathway which regulates cellular metabolism growth and proliferation. Additionally EIF3S2 interacts with the PI3K/Akt pathway coordinating with proteins like mTOR and S6 kinases to control translation initiation and cell survival. These interactions highlight the importance of EIF3S2 in controlling translational efficiency and promoting anabolic processes within the cell.

EIF3S2 has associations with certain cancers such as hepatocellular carcinoma and colorectal cancer. Altered expression of EIF3S2 can lead to disrupted protein synthesis contributing to tumorigenesis and cancer progression. The protein shows a potential link to oncogenic pathways coordinating with mutations or overexpression of related proteins like mTOR and Akt in tumor cells. Understanding EIF3S2's role in these diseases might provide insights for targeted therapies and cancer treatment strategies.
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Specifications

Form

Liquid

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General info

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).

Sequence similarities

Belongs to the eIF-3 subunit I family.

Post-translational modifications

Phosphorylated by TGF-beta type II receptor.

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Product protocols

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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).
See full target information EIF3I
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