Recombinant Human eIF4B protein is a Human Full Length protein, in the 1 to 611 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- Nature communications 10:20712019The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNavdeep Malik et. al.PubMed 31061501
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A S A K K K N K K G K T I S L T D F L A E D G G T G G G S T Y V S K P V S W A D E T D D L E G D V S T T W H S N D D D V Y R A P P I D R S I L P T A P R A A R E P N I D R S R L P K S P P Y T A F L G N L P Y D V T E E S I K E F F R G L N I S A V R L P R E P S N P E R L K G F G Y A E F E D L D S L L S A L S L N E E S L G N R R I R V D V A D Q A Q D K D R D D R S F G R D R N R D S D K T D T D W R A R P A T D S F D D Y P P R R G D D S F G D K Y R D R Y D S D R Y R D G Y R D G Y R D G P C R D M D R Y G G R D R Y D D R G S R D Y D R G Y D S R I G S G R R A F G S G Y R R D D D Y R G G G D R Y E D R Y D R R D D R S W S S R D D Y S R D D Y R R D D R G P P Q R P K L N L K P R S T P K E D D S S A S T S Q S T R A A S I F G G A K P V D T A A R E R E V E E R L Q K E Q E K L Q R Q L D E P K L E R R P R E R H P S W R S E E T Q E R E R S R T G S E S S Q T G T S T T S S R N A R R R E S E K S L E N E T L N K E E D C H S P T S K P P K P D Q P L K V M P A P P P K E N A W V K R S S N P P A R S Q S S D T E Q Q S P T S G G G K V A P A Q P S E E G P G R K D E N K V D G M N A P K G Q T G N S S R G P G D G G N R D H W K E S D R K D G K K D Q D S R S A P E P K K P E E N P A S K F S S A S K Y A A L S V D G E D E N E G E D Y A E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Alternative names
Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
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Recombinant Human eIF4B protein is a Human Full Length protein, in the 1 to 611 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P23588-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M A A S A K K K N K K G K T I S L T D F L A E D G G T G G G S T Y V S K P V S W A D E T D D L E G D V S T T W H S N D D D V Y R A P P I D R S I L P T A P R A A R E P N I D R S R L P K S P P Y T A F L G N L P Y D V T E E S I K E F F R G L N I S A V R L P R E P S N P E R L K G F G Y A E F E D L D S L L S A L S L N E E S L G N R R I R V D V A D Q A Q D K D R D D R S F G R D R N R D S D K T D T D W R A R P A T D S F D D Y P P R R G D D S F G D K Y R D R Y D S D R Y R D G Y R D G Y R D G P C R D M D R Y G G R D R Y D D R G S R D Y D R G Y D S R I G S G R R A F G S G Y R R D D D Y R G G G D R Y E D R Y D R R D D R S W S S R D D Y S R D D Y R R D D R G P P Q R P K L N L K P R S T P K E D D S S A S T S Q S T R A A S I F G G A K P V D T A A R E R E V E E R L Q K E Q E K L Q R Q L D E P K L E R R P R E R H P S W R S E E T Q E R E R S R T G S E S S Q T G T S T T S S R N A R R R E S E K S L E N E T L N K E E D C H S P T S K P P K P D Q P L K V M P A P P P K E N A W V K R S S N P P A R S Q S S D T E Q Q S P T S G G G K V A P A Q P S E E G P G R K D E N K V D G M N A P K G Q T G N S S R G P G D G G N R D H W K E S D R K D G K K D Q D S R S A P E P K K P E E N P A S K F S S A S K Y A A L S V D G E D E N E G E D Y A E
- Accession
- P23588
- Protein length
- Full Length
- Predicted molecular weight
- 93.28 kDa
- Amino acids
- 1 to 611
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
- Post-translational modifications
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. In response to mTORC1 activation, RPS6KA1-mediated phosphorylation at 'Ser-406' and 'Ser-422' stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.
- Biological function summary
EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.
- Pathways
EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.
- Associated diseases and disorders
Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.
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1 product image
SDS-PAGE - Recombinant Human eIF4B protein (ab114511)
ab114511 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.
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