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AB114511

Recombinant Human eIF4B protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human eIF4B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 611 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

1 Images
SDS-PAGE - Recombinant Human eIF4B protein (GST tag N-Terminus) (AB114511)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human eIF4B protein (GST tag N-Terminus) (AB114511)

ab114511 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P23588

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB68474

Anti-eIF4B antibody [EP2299Y]

RabMAb

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

  • 5
  • 1
Proteins & Peptides

AB99961

Recombinant Human PSME1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PSME1 protein (His tag N-Terminus) (AB99961)

  • 0
  • 0
Primary Antibodies

AB235448

Anti-Acid phosphatase antibody [EPR21787]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21787] (AB235448)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPCRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE","proteinLength":"Full Length","predictedMolecularWeight":"93.28 kDa","actualMolecularWeight":null,"aminoAcidEnd":611,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P23588","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.
Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.
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Specifications

Form

Liquid

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General info

Function

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Post-translational modifications

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. In response to mTORC1 activation, RPS6KA1-mediated phosphorylation at 'Ser-406' and 'Ser-422' stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function.

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Product protocols

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Target data

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
See full target information EIF4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:2071 PubMed31061501

2019

The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Navdeep Malik,Hualong Yan,Nellie Moshkovich,Murali Palangat,Howard Yang,Vanesa Sanchez,Zhuo Cai,Tyler J Peat,Shunlin Jiang,Chengyu Liu,Maxwell Lee,Beverly A Mock,Stuart H Yuspa,Daniel Larson,Lalage M Wakefield,Jing Huang
View all publications
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Product promise

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