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AB235068

Recombinant Human eIF4G1 protein (Tagged)

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Recombinant Human eIF4G1 protein (Tagged) is a Human Fragment protein, in the 1250 to 1599 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

EIF4F, EIF4G, EIF4GI, EIF4G1, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

1 Images
SDS-PAGE - Recombinant Human eIF4G1 protein (Tagged) (AB235068)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human eIF4G1 protein (Tagged) (AB235068)

ab235068 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q04637

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN","proteinLength":"Fragment","predictedMolecularWeight":"46.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":1599,"aminoAcidStart":1250,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q04637","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4G1 also known as eukaryotic translation initiation factor 4 gamma 1 plays an important role in protein synthesis by acting as a scaffold for various other components of the initiation complex. This protein physically binds with eIF4E eIF4A and the poly(A)-binding protein aiding in the recruitment of ribosomes to the mRNA. eIF4G1 has a molecular weight of approximately 180 kDa and is expressed in various tissues including brain heart and skeletal muscle.
Biological function summary

EIF4G1 functions as part of the eIF4F complex facilitating the initiation of cap-dependent translation. This process directly affects the synthesis of proteins that are important for growth and cell cycle progression. As an important component in translation eIF4G1 coordinates interactions between mRNA and ribosomes ensuring efficient translation initiation and progression.

Pathways

EIF4G1 is significant in the mTOR and MAPK signaling pathways. These pathways regulate various cellular processes such as cell growth proliferation and survival. eIF4G1 interacts with related proteins like eIF4E which binds the 5' cap of mRNA and eIF4A an RNA helicase both critical in these signaling cascades.

EIF4G1 has been associated with neurodegenerative diseases like Parkinson's disease and certain types of cancer. Mutations or dysregulation can alter protein synthesis rates and lead to the development of disease phenotypes. In Parkinson's disease the interplay between eIF4G1 and other proteins like alpha-synuclein provides insight into the cellular mechanisms underlying disease progression highlighting eIF4G1 as a potential therapeutic target.
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Specifications

Form

Liquid

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General info

Function

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed : 29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed : 29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed : 29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed : 29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed : 29062139).

Sequence similarities

Belongs to the eukaryotic initiation factor 4G family.

Post-translational modifications

Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.

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Product protocols

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Target data

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed : 29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed : 29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed : 29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed : 29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed : 29062139).
See full target information EIF4G1
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