Recombinant Human eIF4G1 protein (Tagged) is a Human Fragment protein, in the 1250 to 1599 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
I E E Y L H L N D M K E A V Q C V Q E L A S P S L L F I F V R H G V E S T L E R S A I A R E H M G Q L L H Q L L C A G H L S T A Q Y Y Q G L Y E I L E L A E D M E I D I P H V W L Y L A E L V T P I L Q E G G V P M G E L F R E I T K P L R P L G K A A S L L L E I L G L L C K S M G P K K V G T L W R E A G L S W K E F L P E G Q D I G A F V A E Q K V E Y T L G E E S E A P G Q R A L P S E E L N R Q L E K L L K E G S S N Q R V F D W I E A N L S E Q Q I V S N T L V R A L M T A V C Y S A I I F E T P L R V D V A V L K A R A K L L Q K Y L C D E Q K E L Q A L Y A L Q A L V V T L E Q P P N L L R M F F D A L Y D E D V V K E D A F Y S W E S S K D P A E Q Q G K G V A L K S V T A F F K W L R E A E E E S D H N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed:29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed:29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed:29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139).
Alternative names
EIF4F, EIF4G, EIF4GI, EIF4G1, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220
Recommended products
Recombinant Human eIF4G1 protein (Tagged) is a Human Fragment protein, in the 1250 to 1599 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q04637-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- I E E Y L H L N D M K E A V Q C V Q E L A S P S L L F I F V R H G V E S T L E R S A I A R E H M G Q L L H Q L L C A G H L S T A Q Y Y Q G L Y E I L E L A E D M E I D I P H V W L Y L A E L V T P I L Q E G G V P M G E L F R E I T K P L R P L G K A A S L L L E I L G L L C K S M G P K K V G T L W R E A G L S W K E F L P E G Q D I G A F V A E Q K V E Y T L G E E S E A P G Q R A L P S E E L N R Q L E K L L K E G S S N Q R V F D W I E A N L S E Q Q I V S N T L V R A L M T A V C Y S A I I F E T P L R V D V A V L K A R A K L L Q K Y L C D E Q K E L Q A L Y A L Q A L V V T L E Q P P N L L R M F F D A L Y D E D V V K E D A F Y S W E S S K D P A E Q Q G K G V A L K S V T A F F K W L R E A E E E S D H N
- Accession
- Q04637
- Protein length
- Fragment
- Predicted molecular weight
- 46.6 kDa
- Amino acids
- 1250 to 1599
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed:29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed:29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed:29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139).
- Sequence similarities
Belongs to the eukaryotic initiation factor 4G family.
- Post-translational modifications
Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF4G1 also known as eukaryotic translation initiation factor 4 gamma 1 plays an important role in protein synthesis by acting as a scaffold for various other components of the initiation complex. This protein physically binds with eIF4E eIF4A and the poly(A)-binding protein aiding in the recruitment of ribosomes to the mRNA. eIF4G1 has a molecular weight of approximately 180 kDa and is expressed in various tissues including brain heart and skeletal muscle.
- Biological function summary
EIF4G1 functions as part of the eIF4F complex facilitating the initiation of cap-dependent translation. This process directly affects the synthesis of proteins that are important for growth and cell cycle progression. As an important component in translation eIF4G1 coordinates interactions between mRNA and ribosomes ensuring efficient translation initiation and progression.
- Pathways
EIF4G1 is significant in the mTOR and MAPK signaling pathways. These pathways regulate various cellular processes such as cell growth proliferation and survival. eIF4G1 interacts with related proteins like eIF4E which binds the 5' cap of mRNA and eIF4A an RNA helicase both critical in these signaling cascades.
- Associated diseases and disorders
EIF4G1 has been associated with neurodegenerative diseases like Parkinson's disease and certain types of cancer. Mutations or dysregulation can alter protein synthesis rates and lead to the development of disease phenotypes. In Parkinson's disease the interplay between eIF4G1 and other proteins like alpha-synuclein provides insight into the cellular mechanisms underlying disease progression highlighting eIF4G1 as a potential therapeutic target.
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1 product image
SDS-PAGE - Recombinant Human eIF4G1 protein (Tagged) (ab235068)
ab235068 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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