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AB126684

Recombinant Human eIF4H protein (His tag N-Terminus)

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Recombinant Human eIF4H protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 248 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KIAA0038, WBSCR1, WSCR1, EIF4H, Eukaryotic translation initiation factor 4H, eIF-4H, Williams-Beuren syndrome chromosomal region 1 protein

1 Images
SDS-PAGE - Recombinant Human eIF4H protein (His tag N-Terminus) (AB126684)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human eIF4H protein (His tag N-Terminus) (AB126684)

15% SDS-PAGE showing ab126684 (3 µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q15056

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGMGSSRESRGGWDSRDDFNSGFRDDFLGGRGGSRPGDRRTGPPMGSRFRDGPPLRGSNMDFREPTEEERAQRPRLQLKPRTVATPLNQVANPNSAIFGGARPREEVVQKEQE","proteinLength":"Full Length","predictedMolecularWeight":"29.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":248,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15056","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 4H commonly known as eIF4H is an essential protein participating in the initiation phase of protein synthesis. It holds a mass of approximately 27 kDa. eIF4H is expressed in various tissues including the brain liver and muscle. The protein promotes the unwinding of RNA secondary structures aiding ribosome attachment to the mRNA. This function aligns closely with its alternate name eukaryotic translation initiation factor 4H.
Biological function summary

EIF4H enhances the helicase activity of eIF4A an RNA helicase vital for translation initiation. It forms part of the translation initiation complex. The complex brings together eIF4E eIF4G and eIF4A enhancing mRNA recognition and processing. eIF4H stabilizes the eIF4A-mRNA interaction reducing the likelihood of premature dissociation and permitting efficient scanning for the start codon.

Pathways

EIF4H plays a significant role in the mTOR (mechanistic target of rapamycin) signaling pathway which regulates cell growth and proliferation. This pathway influences protein synthesis by controlling the availability and activity of initiation factors like eIF4A and eIF4E. mTOR signaling overlaps with the MAPK (mitogen-activated protein kinase) pathway with eIF4H indirectly interacting with RAF proteins which regulate cell cycle progression and differentiation.

Researchers link eIF4H to neurological disorders like Autism Spectrum Disorder and certain cancers such as glioblastoma. Alterations in the expression or function of eIF4H may impact the mTOR pathway influencing tumor progression and neural development. These dysfunctional roles also involve eIF4E as both proteins contribute to abnormal translation initiation and gene expression in these conditions.
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Specifications

Form

Liquid

Additional notes

ab126684 is purified using conventional chromatography techniques.

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General info

Function

Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.

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Product protocols

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Target data

Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
See full target information EIF4H
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