Recombinant Human ELAC1 protein is a Human Full Length protein, in the 1 to 363 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S M D V T F L G T G A A Y P S P T R G A S A V V L R C E G E C W L F D C G E G T Q T Q L M K S Q L K A G R I T K I F I T H L H G D H F F G L P G L L C T I S L Q S G S M V S K Q P I E I Y G P V G L R D F I W R T M E L S H T E L V F H Y V V H E L V P T A D Q C P A E E L K E F A H V N R A D S P P K E E Q G R T I L L D S E E N S Y L L F D D E Q F V V K A F R L F H R I P S F G F S V V E K K R P G K L N A Q K L K D L G V P P G P A Y G K L K N G I S V V L E N G V T I S P Q D V L K K P I V G R K I C I L G D C S G V V G D G G V K L C F E A D L L I H E A T L D D A Q M D K A K E H G H S T P Q M A A T F A K L C R A K R L V L T H F S Q R Y K P V A L A R E G E T D G I A E L K K Q A E S V L D L Q E V T L A E D F M V I S I P I K K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity (PubMed:12711671, PubMed:32075755). Specifically involved in tRNA repair: acts downstream of the ribosome-associated quality control (RQC) pathway by removing a 2',3'-cyclic phosphate from tRNAs following cleavage by ANKZF1 (PubMed:32075755). tRNAs are then processed by TRNT1 (PubMed:32075755).
Alternative names
D29, ELAC1, Zinc phosphodiesterase ELAC protein 1, Deleted in Ma29, ElaC homolog protein 1, Ribonuclease Z 1, tRNA 3 endonuclease 1, tRNase Z 1, RNase Z 1
Recombinant Human ELAC1 protein is a Human Full Length protein, in the 1 to 363 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9H777-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S M D V T F L G T G A A Y P S P T R G A S A V V L R C E G E C W L F D C G E G T Q T Q L M K S Q L K A G R I T K I F I T H L H G D H F F G L P G L L C T I S L Q S G S M V S K Q P I E I Y G P V G L R D F I W R T M E L S H T E L V F H Y V V H E L V P T A D Q C P A E E L K E F A H V N R A D S P P K E E Q G R T I L L D S E E N S Y L L F D D E Q F V V K A F R L F H R I P S F G F S V V E K K R P G K L N A Q K L K D L G V P P G P A Y G K L K N G I S V V L E N G V T I S P Q D V L K K P I V G R K I C I L G D C S G V V G D G G V K L C F E A D L L I H E A T L D D A Q M D K A K E H G H S T P Q M A A T F A K L C R A K R L V L T H F S Q R Y K P V A L A R E G E T D G I A E L K K Q A E S V L D L Q E V T L A E D F M V I S I P I K K
- Accession
- Q9H777
- Protein length
- Full Length
- Predicted molecular weight
- 42.4 kDa
- Amino acids
- 1 to 363
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab201882 was purified by using conventional chromatography techniques.
General info
- Function
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity (PubMed:12711671, PubMed:32075755). Specifically involved in tRNA repair: acts downstream of the ribosome-associated quality control (RQC) pathway by removing a 2',3'-cyclic phosphate from tRNAs following cleavage by ANKZF1 (PubMed:32075755). tRNAs are then processed by TRNT1 (PubMed:32075755).
- Sequence similarities
Belongs to the RNase Z family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELAC1 also known as ElaC ribonuclease Z 1 is an enzyme involved mechanically in the processing of tRNA. It functions by endonucleolytically cleaving tRNA precursors to produce mature tRNA molecules. This protein has an approximate mass of 93 kDa and exhibits expression in various human tissues with higher levels in the liver and brain. ELAC1 acts as a zinc-dependent peptidase and shares structural similarities with the family of RNase Z enzymes.
- Biological function summary
ELAC1 plays a significant role in tRNA maturation and processing. It participates in the cleavage and removal of the 3' trailer from pre-tRNA molecules. Although not all tRNA processing events require ELAC1 its role is important where it is involved ensuring proper formation of tRNA 3' termini. ELAC1 does not form a part of larger complexes like its homolog ELAC2 which participates in mitochondrial RNA processing. It maintains its activity independently indicating specificity in its function.
- Pathways
ELAC1's function integrates into the broader tRNA processing pathway. This pathway is essential for proper translation and protein synthesis across eukaryotic cells. ELAC1 operates independently within these pathways but works in concert with other processing enzymes such as enzymes involved in tRNA splicing. It facilitates the maturation process that enables proper recognition and usage of tRNA by the ribosome during protein synthesis.
- Associated diseases and disorders
ELAC1 associates with certain neurological conditions due to its expression in brain tissues. Variations in ELAC1 activity or expression could potentially contribute to disorders such as Alzheimer's disease. The relationship between ELAC1 and mitochondrial proteins like ELAC2 suggests potential overlapping contributions to mitochondrial dysfunctions observed in various neurodegenerative diseases. Further research may help elucidate the exact role that ELAC1 and its interaction partners play in disease development and progression.
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1 product image
SDS-PAGE - Recombinant Human ELAC1 protein (ab201882)
15% SDS PAGE analysis of ab201882 (3 μg).
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