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AB201882

Recombinant Human ELAC1 protein

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Recombinant Human ELAC1 protein is a Human Full Length protein, in the 1 to 363 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

D29, ELAC1, Zinc phosphodiesterase ELAC protein 1, Deleted in Ma29, ElaC homolog protein 1, Ribonuclease Z 1, tRNA 3 endonuclease 1, tRNase Z 1, RNase Z 1

1 Images
SDS-PAGE - Recombinant Human ELAC1 protein (AB201882)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ELAC1 protein (AB201882)

15% SDS PAGE analysis of ab201882 (3 μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9H777

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSMDVTFLGTGAAYPSPTRGASAVVLRCEGECWLFDCGEGTQTQLMKSQLKAGRITKIFITHLHGDHFFGLPGLLCTISLQSGSMVSKQPIEIYGPVGLRDFIWRTMELSHTELVFHYVVHELVPTADQCPAEELKEFAHVNRADSPPKEEQGRTILLDSEENSYLLFDDEQFVVKAFRLFHRIPSFGFSVVEKKRPGKLNAQKLKDLGVPPGPAYGKLKNGISVVLENGVTISPQDVLKKPIVGRKICILGDCSGVVGDGGVKLCFEADLLIHEATLDDAQMDKAKEHGHSTPQMAATFAKLCRAKRLVLTHFSQRYKPVALAREGETDGIAELKKQAESVLDLQEVTLAEDFMVISIPIKK","proteinLength":"Full Length","predictedMolecularWeight":"42.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":363,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H777","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELAC1 also known as ElaC ribonuclease Z 1 is an enzyme involved mechanically in the processing of tRNA. It functions by endonucleolytically cleaving tRNA precursors to produce mature tRNA molecules. This protein has an approximate mass of 93 kDa and exhibits expression in various human tissues with higher levels in the liver and brain. ELAC1 acts as a zinc-dependent peptidase and shares structural similarities with the family of RNase Z enzymes.
Biological function summary

ELAC1 plays a significant role in tRNA maturation and processing. It participates in the cleavage and removal of the 3' trailer from pre-tRNA molecules. Although not all tRNA processing events require ELAC1 its role is important where it is involved ensuring proper formation of tRNA 3' termini. ELAC1 does not form a part of larger complexes like its homolog ELAC2 which participates in mitochondrial RNA processing. It maintains its activity independently indicating specificity in its function.

Pathways

ELAC1's function integrates into the broader tRNA processing pathway. This pathway is essential for proper translation and protein synthesis across eukaryotic cells. ELAC1 operates independently within these pathways but works in concert with other processing enzymes such as enzymes involved in tRNA splicing. It facilitates the maturation process that enables proper recognition and usage of tRNA by the ribosome during protein synthesis.

ELAC1 associates with certain neurological conditions due to its expression in brain tissues. Variations in ELAC1 activity or expression could potentially contribute to disorders such as Alzheimer's disease. The relationship between ELAC1 and mitochondrial proteins like ELAC2 suggests potential overlapping contributions to mitochondrial dysfunctions observed in various neurodegenerative diseases. Further research may help elucidate the exact role that ELAC1 and its interaction partners play in disease development and progression.
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Specifications

Form

Liquid

Additional notes

ab201882 was purified by using conventional chromatography techniques.

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General info

Function

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity (PubMed : 12711671, PubMed : 32075755). Specifically involved in tRNA repair : acts downstream of the ribosome-associated quality control (RQC) pathway by removing a 2',3'-cyclic phosphate from tRNAs following cleavage by ANKZF1 (PubMed : 32075755). tRNAs are then processed by TRNT1 (PubMed : 32075755).

Sequence similarities

Belongs to the RNase Z family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity (PubMed : 12711671, PubMed : 32075755). Specifically involved in tRNA repair : acts downstream of the ribosome-associated quality control (RQC) pathway by removing a 2',3'-cyclic phosphate from tRNAs following cleavage by ANKZF1 (PubMed : 32075755). tRNAs are then processed by TRNT1 (PubMed : 32075755).
See full target information ELAC1
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