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AB158353

Recombinant Human ELK4 protein (GST tag N-Terminus)

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Recombinant Human ELK4 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 405 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

SAP1, ELK4, ETS domain-containing protein Elk-4, Serum response factor accessory protein 1, SAP-1, SRF accessory protein 1

1 Images
SDS-PAGE - Recombinant Human ELK4 protein (GST tag N-Terminus) (AB158353)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ELK4 protein (GST tag N-Terminus) (AB158353)

ab158353 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P28324

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB86002

Anti-ELK4 antibody

Western blot - Anti-ELK4 antibody (AB86002)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDSAITLWQFLLQLLQKPQNKHMICWTSNDGQFKLLQAEEVARLWGIRKNKPNMNYDKLSRALRYYYVKNIIKKVNGQKFVYKFVSYPEILNMDPMTVGRIEGDCESLNFSEVSSSSKDVENGGKDKPPQPGAKTSSRNDYIHSGLYSSFTLNSLNSSNVKLFKLIKTENPAEKLAEKKSPQEPTPSVIKFVTTPSKKPPVEPVAATISIGPSISPSSEETIQALETLVSPKLPSLEAPTSASNVMTAFATTPPISSIPPLQEPPRTPSPPLSSHPDIDTDIDSVASQPMELPENLSLEPKDQDSVLLEKDKVNNSSRSKKPKGLELAPTLVITSSDPSPLGILSPSLPTASLTPAFFSQVACSLFMVSPLLSFICPFKQIQNLYTQVCFLLLRFVLERLCVTVM","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":405,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P28324","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELK4 also known as SAP-1 (Serum Response Factor Accessory Protein 1) functions as a transcription factor within the Ets family. ELK4's molecular mass is approximately 51 kDa. This protein is expressed widely in various tissues including the brain heart and lungs. It binds to the DNA sequence GGAA/T to regulate gene expression and plays a significant role in cellular processes by modulating transcriptional response to external stimuli.
Biological function summary

The ELK4 protein influences cell proliferation and differentiation. It forms a complex with serum response factor (SRF) to modulate the activity of immediate early genes like c-fos. The ELK4-SRF complex binds to serum response elements (SREs) within promoter regions of target genes thereby impacting cellular responses such as growth and survival. Additionally ELK4's interactions with other proteins in the nucleus enhance its function in regulating gene expression.

Pathways

The ELK4 protein plays an important role in the MAPK/ERK signaling pathway which is important for transmitting extracellular signals to the nucleus and affecting gene expression. It interacts closely with other proteins such as ERK1/2 leading to its phosphorylation and activation. Also ELK4 participates in the Ras signaling cascade further influencing cellular responses to growth factors.

ELK4's dysfunctional regulation contributes to cancer particularly in prostate carcinoma. Aberrant ELK4 signaling affects the expression of genes related to cell proliferation and apoptosis supporting tumorigenesis. Additionally ELK4 associates with the SRF protein in the development of cardiovascular disorders where anomalous signaling can lead to pathological cardiac hypertrophy and failure.
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Specifications

Form

Liquid

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General info

Function

Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.

Sequence similarities

Belongs to the ETS family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
See full target information ELK4
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Product promise

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