Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) is a Human Full Length protein, in the 2 to 212 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
P R R A E N W D E A E V G A E E A G V E E Y G P E E D G G E E S G A E E S G P E E S G P E E L G A E E E M E A G R P R P V L R S V N S R E P S Q V I F C N R S P R V V L P V W L N F D G E P Q P Y P T L P P G T G R R I H S Y R G H L W L F R D A G T H D G L L V N Q T E L F V P S L N V D G Q P I F A N I T L P V Y T L K E R C L Q V V R S L V K P E N Y R R L D I V R S L Y E D L E D H P N V Q K D L E R L T Q E R I A H Q R M G D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed:7638163). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity). Core component of multiple cullin-2 and cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes), which mediate the ubiquitination of target proteins (PubMed:10205047, PubMed:12004076, PubMed:12050673, PubMed:15590694, PubMed:26138980, PubMed:29775578, PubMed:29779948, PubMed:33268465, PubMed:38326650). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes (PubMed:10205047, PubMed:12004076, PubMed:12050673, PubMed:15590694). Component the von Hippel-Lindau ubiquitination complex CBC(VHL) (PubMed:10205047, PubMed:12004076, PubMed:12050673, PubMed:15590694). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:26138980, PubMed:29775578, PubMed:29779948). The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (PubMed:33268465). As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A (PubMed:19920177). ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (By similarity). (Microbial infection) Following infection by HIV-1 virus, component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, which catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed:18562529, PubMed:20532212, PubMed:22190037, PubMed:24225024, PubMed:24402281, PubMed:36754086). The complex can also ubiquitinate APOBEC3H to some extent (PubMed:37640699).
Additional Targets
ELOC, VHL
Alternative names
TCEB2, ELOB, Elongin-B, EloB, Elongin 18 kDa subunit, RNA polymerase II transcription factor SIII subunit B, SIII p18, Transcription elongation factor B polypeptide 2
Recommended products
Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) is a Human Full Length protein, in the 2 to 212 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Assay Conditions: Various amounts of ab271497 were incubated in 10-ul reaction with ARV-771 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
- Accession
- Q15370-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- P R R A E N W D E A E V G A E E A G V E E Y G P E E D G G E E S G A E E S G P E E S G P E E L G A E E E M E A G R P R P V L R S V N S R E P S Q V I F C N R S P R V V L P V W L N F D G E P Q P Y P T L P P G T G R R I H S Y R G H L W L F R D A G T H D G L L V N Q T E L F V P S L N V D G Q P I F A N I T L P V Y T L K E R C L Q V V R S L V K P E N Y R R L D I V R S L Y E D L E D H P N V Q K D L E R L T Q E R I A H Q R M G D
- Accession
- P40337
- Protein length
- Full Length
- Predicted molecular weight
- 25 kDa
- Amino acids
- 2 to 212
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 2
- D V F L M I R R H K T T I F T D A K E S S T V F E L K R I V E G I L K R P P D E Q R L Y K D D Q L L D D G K T L G E C G F T S Q T A R P Q A P A T V G L A F R A D D T F E A L C I E P F S S P P E L P D V M K P Q D S G S S A N E Q A V Q
- Accession
- Q15370
- Protein length
- Full Length
- Predicted molecular weight
- 14 kDa
- Amino acids
- 2 to 118
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 3
- D G E E K T Y G G C E G P D A M Y V K L I S S D G H E F I V K R E H A L T S G T I K A M L S G P G Q F A E N E T N E V N F R E I P S H V L S K V C M Y F T Y K V R Y T N S S T E I P E F P I A P E I A L E L L M A A N F L D C
- Accession
- Q15369
- Protein length
- Full Length
- Predicted molecular weight
- 13 kDa
- Amino acids
- 2 to 112
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed:7638163). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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2 product images
Functional Studies - Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) (ab271497)
ab271497-BRD3 interaction.
SDS-PAGE - Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) (ab271497)
SDS-PAGE analysis of ab271497.
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