Recombinant Human Elongin-C protein (His tag N-Terminus)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human Elongin-C protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 112 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
TCEB1, ELOC, Elongin-C, EloC, Elongin 15 kDa subunit, RNA polymerase II transcription factor SIII subunit C, SIII p15, Transcription elongation factor B polypeptide 1
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Elongin-C protein (His tag N-Terminus) (AB116195)
15% SDS-PAGE analysis of 3 µg ab116195.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Elongin C participates in stabilizing the association of Elongin A with RNA polymerase II. It is an essential component of the transcription elongation complex influencing the transcriptional activity of genes. By forming a complex with Elongin B it propels the transcription process forward particularly in response to signals that regulate transcription elongation. Elongin C also connects with the cullin-RING E3 ubiquitin ligase (CRL) complex contributing to protein degradation pathways.
Pathways
Elongin C plays a significant role in the ubiquitin-proteasome system which targets proteins for degradation. It functions alongside Elongin A and Elongin B in this pathway where these components coordinate to control protein levels in the cell. Elongin C associates with the VHL (Von Hippel-Lindau) tumor suppressor protein in the pathway responsible for hypoxia-inducible factor (HIF) degradation therefore influencing cellular responses to oxygen availability.
Specifications
Form
Liquid
Additional notes
ab116195 was purified using conventional chromatography techniques.
General info
Function
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed : 7821821). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity).. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins (PubMed : 10205047, PubMed : 12004076, PubMed : 12050673, PubMed : 15590694, PubMed : 21199876, PubMed : 26138980, PubMed : 29775578, PubMed : 29779948, PubMed : 30166453, PubMed : 33268465, PubMed : 38326650). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes (PubMed : 10205047, PubMed : 12004076, PubMed : 12050673, PubMed : 15590694). Component the von Hippel-Lindau ubiquitination complex CBC(VHL) (PubMed : 10205047, PubMed : 12004076, PubMed : 12050673, PubMed : 15590694). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed : 26138980, PubMed : 29775578, PubMed : 29779948). The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (PubMed : 33268465). As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A (PubMed : 19920177). ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (By similarity).. (Microbial infection) Following infection by HIV-1 virus, component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, which catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed : 18562529, PubMed : 20532212, PubMed : 22190037, PubMed : 24225024, PubMed : 24402281, PubMed : 36754086). The complex can also ubiquitinate APOBEC3H to some extent (PubMed : 37640699).
Sequence similarities
Belongs to the SKP1 family.
Post-translational modifications
Ubiquitinated by the DCX(AMBRA1) complex, leading to its degradation by the proteasome.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com