Recombinant Human ELOVL4 protein is a Human Fragment protein, in the 99 to 154 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M G S Y N A G Y S Y I C Q S V D Y S N N V H E V R I A A A L W W Y F V S K G V E Y L D T V F F I L R K K N N Q V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.
Alternative names
Very long chain fatty acid elongase 4, 3-keto acyl-CoA synthase ELOVL4, ELOVL fatty acid elongase 4, Elongation of very long chain fatty acids protein 4, Very long chain 3-ketoacyl-CoA synthase 4, Very long chain 3-oxoacyl-CoA synthase 4, ELOVL FA elongase 4, ELOVL4
Recommended products
Recombinant Human ELOVL4 protein is a Human Fragment protein, in the 99 to 154 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9GZR5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M G S Y N A G Y S Y I C Q S V D Y S N N V H E V R I A A A L W W Y F V S K G V E Y L D T V F F I L R K K N N Q V
- Accession
- Q9GZR5
- Protein length
- Fragment
- Amino acids
- 99 to 154
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.
- Sequence similarities
Belongs to the ELO family. ELOVL4 subfamily.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELOVL4 or Elongation of Very Long Chain Fatty Acids Protein 4 plays a critical role in the biosynthesis of very long chain fatty acids. It weighs approximately 32 kDa. It elongates saturated and polyunsaturated fatty acids extending carbon chains to lengths exceeding 24 carbons. ELOVL4 is found mainly in the retina skin and brain reflecting its significance in these tissues. Its mechanical function involves adding two carbons per cycle to endogenous and dietary fatty acids substrates.
- Biological function summary
This enzyme contributes to the production of essential lipids that maintain cellular structure and function. ELOVL4 integrates into larger protein complexes that regulate its activity or stabilize its function. It influences the quality of long-chain ceramides and other sphingolipids which impact barrier functions particularly in the skin and retina. Moreover it participates in maintaining membrane integrity and dynamics where these specialized lipids concentrate.
- Pathways
ELOVL4 participates prominently in sphingolipid and lipid metabolism pathways. It interacts with other elongases and desaturases such as ELOVL1 to execute its elongation tasks. This protein's activity bears importance in both the synthesis and modification phases of very long-chain fatty acids critical to cellular processes. The sphingolipid pathway necessitates ELOVL4 in producing functional lipids ensuring proper membrane properties and signaling events.
- Associated diseases and disorders
Mutations in the ELOVL4 gene have links to conditions like Stargardt-like macular dystrophy and ichthyosis. Stargardt disease involves retinal degeneration due to disruptions in photoreceptor cell function attributed to defective fatty acid elongation. In skin-related disorders like ichthyosis ELOVL4 mutations disturb lipid processing affecting skin barrier formation. Pathologically its dysfunction associates with other lipogenic and barrier-forming proteins including ceramide synthases impacting the synthesis of necessary lipid components.
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1 product image
SDS-PAGE - Recombinant Human ELOVL4 protein (ab159600)
ab159600 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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