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AB276462

Recombinant Human EMA protein (Fc Chimera)

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Recombinant Human EMA protein (Fc Chimera) is a Human Fragment protein, in the 1 to 167 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human EMA protein (Fc Chimera) (AB276462)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EMA protein (Fc Chimera) (AB276462)

SDS-PAGE analysis of ab276462

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P15941-11

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MTPGTQSPFFLLLLLTVLTATTAPKPATVVTGSGHASSTPGGEKETSATQRSSVPSSTEKNAFNSSLEDPSTDYYQELQRDISEMFLQIYKQGGFLGLSNIKFRPGSVVVQLTLAFREGTINVHDVETQFNQYKTEAASRYNLTISDVSVSDVPFPFSAQSGAGVPG","proteinLength":"Fragment","predictedMolecularWeight":"42 kDa","actualMolecularWeight":null,"aminoAcidEnd":167,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P15941","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMA or epithelial membrane antigen is a well-studied glycoprotein with an approximate molecular weight of 40-60 kDa. Also known as MUC1 this protein predominantly appears on the apical surface of epithelial cells in tissues like breast lung and gastrointestinal tract. EMA exists in abundance on the epithelial cell surface especially in secretory epithelia acting as a molecular barrier between environment and underlying tissue.
Biological function summary

Epithelial membrane antigen plays a role in cell signaling and protection. It is a part of the mucin family which are high-molecular-weight glycoproteins involved in forming protective mucous barriers on epithelial surfaces. EMA has an extended structure that contributes to providing cellular adhesion through its tandem repeat domains. This protein is not typically part of larger multiprotein complexes but nonetheless influences cellular phenotype and immune responses.

Pathways

Epithelial membrane antigen is involved in several key biological processes such as cell adhesion and signal transduction. It plays a critical role in pathways associated with epithelial cell growth and differentiation. An important pathway involving MUC1 is the EGFR signaling pathway where it modulates the activity through interactions with related proteins like EGFR and c-Src. These interactions enable cells to respond to extracellular signals ultimately influencing proliferation and survival.

Epithelial membrane antigen shows a significant correlation with cancer particularly breast and ovarian cancer. Aberrant expression or glycosylation of EMA can lead to altered cell signaling and adhesion promoting tumor progression. MUC1 associates with proteins like p53 and β-catenin during oncogenic transformation which are well-known players in cancer biology. As such EMA serves as an important biomarker for cancer diagnosis and prognosis aiding in the development of therapeutic strategies.
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Product protocols

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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
See full target information MUC1

Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA
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Product promise

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